Linked Life Data

2067533

Source:http://linkedlifedata.com/resource/pubmed/id/2067533

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-8-9
pubmed:abstractText
A mixture of two benzene metabolites, hydroquinone and catechol, produces a striking synergistic genotoxic response in cultured human lymphocytes. This was demonstrated using an anti-kinetochore antibody modification of the micronucleus assay. Treatment with hydroquinone alone or in combination with phenol produced a 3-fold increase in micronucleated cells over background. Treatment with catechol or phenol alone and in combination produced only minor increases in the number of micronucleated cells. In contrast, simultaneous treatment with equimolar (75 microM) concentrations of hydroquinone and catechol resulted in a greater than 16-fold induction of micronucleated cells. Given an additivity model, 20 additional micronucleated cells would be expected (after correcting for background frequencies), yet 140 were observed. Further analysis revealed that over 90% of the micronucleated cells stained positively for kinetochores, indicating a high probability that these micronuclei contain entire chromosomes. This synergistic response appears to occur only at equimolar levels of hydroquinone and catechol. These results suggest that these metabolites are acting together to disrupt the mitotic spindle and interfere with chromosome segregation. These data provide further support for the hypothesis that multiple metabolites acting in concert are involved in the benzene-induced genotoxicity and leukemia in humans.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
249
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Two benzene metabolites, catechol and hydroquinone, produce a synergistic induction of micronuclei and toxicity in cultured human lymphocytes.
pubmed:affiliation
Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.