Linked Life Data

20671291

Source:http://linkedlifedata.com/resource/pubmed/id/20671291

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-7-30
pubmed:abstractText
An increasing amount of evidence demonstrates the beneficial role of oxytocin (OT) in the cardiovascular system. Similar actions are attributed to genistein, an isoflavonic phytoestrogen. The treatment with genistein activates the OT system in the aorta of ovariectomized (OVX) Sprague-Dawley (SD) rats. The objective of this study was to determine the effects of low doses of genistein on the OT-induced effects in rat hypertension. The hypothesis tested was that treatment of OVX spontaneously hypertensive rats (SHRs) with genistein improves heart structure and heart work through a mechanism involving the specific OT receptor (OTR). OVX SHRs or SD rats were treated with genistein (in microg/g body wt sc, 10 days) in the presence or absence of an OT antagonist (OTA) [d(CH(2))(5), Tyr(Me)(2), Orn(8)]-vasotocin or a nonspecific estrogen receptor antagonist (ICI-182780). Vehicle-treated OVX rats served as controls. RT-PCR and Western blot analysis demonstrated that left ventricular (LV) OTR, downregulated by ovariectomy, increased in response to genistein. In SHRs or SD rats, this effect was blocked by OTA or ICI-182780 administration. The OTR was mainly localized in microvessels expressing the CD31 marker and colocalized with endothelial nitric oxide synthase. In SHRs, the genistein-stimulated OTR increases were associated with improved fractional shortening, decreased blood pressure (12 mmHg), decreased heart weight-to-body weight ratio, decreased fibrosis, and lowered brain natriuretic peptide in the LV. The prominent finding of the study is the detrimental effect of OTA treatment on the LV of SHRs. OTA treatment of OVX SHRs resulted in a dramatic worsening of ejection fractions and an augmented fibrosis. In conclusion, these results demonstrate that cardiac OTRs are involved in the regulation of cardiac function of OVX SHRs. The decreases of OTRs may contribute to cardiac pathology following menopause.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1522-1539
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
299
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H265-74
pubmed:meshHeading
pubmed-meshheading:20671291-Animals, pubmed-meshheading:20671291-Atrial Natriuretic Factor, pubmed-meshheading:20671291-Cardiomegaly, pubmed-meshheading:20671291-Disease Models, Animal, pubmed-meshheading:20671291-Dose-Response Relationship, Drug, pubmed-meshheading:20671291-Estradiol, pubmed-meshheading:20671291-Estrogen Antagonists, pubmed-meshheading:20671291-Female, pubmed-meshheading:20671291-Fibrosis, pubmed-meshheading:20671291-Genistein, pubmed-meshheading:20671291-Hypertension, pubmed-meshheading:20671291-Myocardial Contraction, pubmed-meshheading:20671291-Myocardium, pubmed-meshheading:20671291-Natriuretic Peptide, Brain, pubmed-meshheading:20671291-Ovariectomy, pubmed-meshheading:20671291-RNA, Messenger, pubmed-meshheading:20671291-Rats, pubmed-meshheading:20671291-Rats, Inbred SHR, pubmed-meshheading:20671291-Rats, Sprague-Dawley, pubmed-meshheading:20671291-Receptors, Estrogen, pubmed-meshheading:20671291-Receptors, Oxytocin, pubmed-meshheading:20671291-Vasotocin, pubmed-meshheading:20671291-Ventricular Function, Left, pubmed-meshheading:20671291-Ventricular Pressure, pubmed-meshheading:20671291-Ventricular Remodeling
pubmed:year
2010
pubmed:articleTitle
Cardiac oxytocin receptor blockade stimulates adverse cardiac remodeling in ovariectomized spontaneously hypertensive rats.
pubmed:affiliation
Centre de recherche, CHUM-Hôtel-Dieu Pav. De Boulion, 3840, rue Saint-Urbain, Montréal, QC, H2W 1T8, Canada. marek.jankowski@umontreal.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't