Linked Life Data

20665543

Source:http://linkedlifedata.com/resource/pubmed/id/20665543

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-10-27
pubmed:abstractText
The level of circulating endotoxin is related to the severity of cardiovascular disease. One of the indexes for the prognosis of cardiovascular disease is the plasma aldosterone level. Recently, the Toll-like receptors (TLRs), lipopolysaccharide (LPS)-regulated receptors, were found not only to mediate the inflammatory response but also to be important in the adrenal stress response. Whether LPS via TLRs induced aldosterone production in adrenal zona glomerulosa (ZG) cells was not clear. Our results suggest that LPS-induced aldosterone secretion in a time- and dose-dependent manner and via TLR2 and TLR4 signaling pathway. Administration of LPS can enhance steroidogenesis enzyme expression such as scavenger receptor-B1 (SR-B1), steroidogenic acute regulatory protein (StAR) and P450 side chain cleavage (P450scc) enzyme. LPS-induced SR-B1 and StAR protein expression are abolished by TLR2 blocker. Furthermore, we demonstrated that phosphorylation of Akt was elevated by LPS treatment and reduced by TLR2 blockers, TLR4 blockers, and LY294002 (PI(3)K inhibitor). Those inhibitors of PI(3)K/Akt pathways also abolish LPS-induced aldosterone secretion and SR-B1 protein level. In conclusion, LPS-induced aldosterone production and SR-B1 proteins expression are through the TLR2 and TLR4 related PI(3)K/Akt pathways in adrenal ZG cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1097-4644
pubmed:author
pubmed:copyrightInfo
© 2010 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
872-80
pubmed:meshHeading
pubmed-meshheading:20665543-Aldosterone, pubmed-meshheading:20665543-Animals, pubmed-meshheading:20665543-Cholesterol Side-Chain Cleavage Enzyme, pubmed-meshheading:20665543-Lipopolysaccharides, pubmed-meshheading:20665543-Male, pubmed-meshheading:20665543-Models, Biological, pubmed-meshheading:20665543-Phosphatidylinositol 3-Kinases, pubmed-meshheading:20665543-Phosphoproteins, pubmed-meshheading:20665543-Phosphorylation, pubmed-meshheading:20665543-Proto-Oncogene Proteins c-akt, pubmed-meshheading:20665543-Rats, pubmed-meshheading:20665543-Rats, Sprague-Dawley, pubmed-meshheading:20665543-Scavenger Receptors, Class B, pubmed-meshheading:20665543-Signal Transduction, pubmed-meshheading:20665543-Toll-Like Receptor 2, pubmed-meshheading:20665543-Toll-Like Receptor 4, pubmed-meshheading:20665543-Zona Glomerulosa
pubmed:year
2010
pubmed:articleTitle
Lipopolysaccharide directly stimulates aldosterone production via toll-like receptor 2 and toll-like receptor 4 related PI(3)K/Akt pathway in rat adrenal zona glomerulosa cells.
pubmed:affiliation
Department and Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
pubmed:publicationType
Journal Article