|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2010-9-14
|
|
pubmed:abstractText |
The stereo-specific L-isoleucine-4-hydroxylase (L-isoleucine dioxygenase (IDO)) was cloned and expressed in an Escherichia coli 2? strain lacking the activities of ?-ketoglutarate dehydrogenase (EC 1.2.4.2), isocitrate liase (EC 4.1.3.1), and isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5). The 2? strain could not grow in a minimal-salt/glucose/glycerol medium due to the blockage of TCA during succinate synthesis. The IDO activity in the 2? strain was able to "shunt" destroyed TCA, thereby coupling L-isoleucine hydroxylation and cell growth. Using this strain, we performed the direct biotransformation of L-isoleucine into 4-HIL with an 82% yield.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
1432-0614
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
88
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
719-26
|
|
pubmed:meshHeading |
pubmed-meshheading:20665018-Amino Acid Sequence,
pubmed-meshheading:20665018-Base Sequence,
pubmed-meshheading:20665018-Biotransformation,
pubmed-meshheading:20665018-Cloning, Molecular,
pubmed-meshheading:20665018-Dioxygenases,
pubmed-meshheading:20665018-Escherichia coli,
pubmed-meshheading:20665018-Fermentation,
pubmed-meshheading:20665018-Gene Expression Regulation, Bacterial,
pubmed-meshheading:20665018-Isoleucine,
pubmed-meshheading:20665018-Ketoglutarate Dehydrogenase Complex,
pubmed-meshheading:20665018-Molecular Sequence Data
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine.
|
|
pubmed:affiliation |
Ajinomoto-Genetika Research Institute, 1st Dorozhny Pr. 1, Moscow 117545, Russia. servasmi@mail.ru
|
|
pubmed:publicationType |
Journal Article
|
