Source:http://linkedlifedata.com/resource/pubmed/id/20664962
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-7-28
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| pubmed:abstractText |
External microwave (EMW) hyperthermia system (2.45 GHz wave frequency) was evaluated by in vitro studies and in vivo pleural metastasis animal model. Three different non-small-cell lung cancer cells and normal fibroblast cells (control) were treated once a day for 3 days with the prototype EMW system applying mild (39 degrees C), moderate (43 degrees C), and severe (47 degrees C) hyperthermia. On Day-4, tested cells were retrieved and examined by apoptosis assay kit and Western blot analysis. Cancer cells treated with moderate hyperthermia showed significant apoptosis; yet no major damage was observed to normal fibroblast cells. Western blot analysis indicated cleavage on caspase-3, -9 and PARP. Also in the cell cycle analysis, increase of sub G0-G1 population was identified. After optimization of the heating intensity for in vivo environment, we created pleural metastatic animal model in 24 immune deficiency mice (male nu/nu mice) to evaluate inhibitory effect of systemic EMW hyperthermia for disseminated tumor growth. Out of 24 mice, 8 received mild and 8 received moderate hyperthermia, and remaining 8 were the no treatment control. Whole chest area of the experimental animals was irradiated 3 times a week for 2 weeks (total of 6 time irradiations). No significant adverse event was observed including abnormal weight loss, skin burn, ulceration, and death. Metastasized tumors around the pleura and chest cavity were 75% reduced in size and weight compared to non-treated control group. Harvested tumors were stained and TUNEL assay demonstrated significant apoptosis in a moderate hyperthermia group. The EMW hyperthermia system may be possible alternative tool as a systemic hyperthermia therapy in severely advanced lung cancer patients. Further study is necessary to determine device safeness, efficacy, and synergistic effect to other possible combination therapies.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1791-2431
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
24
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
591-8
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| pubmed:meshHeading |
pubmed-meshheading:20664962-Animals,
pubmed-meshheading:20664962-Apoptosis,
pubmed-meshheading:20664962-Blotting, Western,
pubmed-meshheading:20664962-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:20664962-Cell Cycle,
pubmed-meshheading:20664962-Cell Line, Tumor,
pubmed-meshheading:20664962-Cell Proliferation,
pubmed-meshheading:20664962-DNA Damage,
pubmed-meshheading:20664962-Humans,
pubmed-meshheading:20664962-Hyperthermia, Induced,
pubmed-meshheading:20664962-In Situ Nick-End Labeling,
pubmed-meshheading:20664962-Lung Neoplasms,
pubmed-meshheading:20664962-Male,
pubmed-meshheading:20664962-Mice,
pubmed-meshheading:20664962-Mice, Nude,
pubmed-meshheading:20664962-Microwaves,
pubmed-meshheading:20664962-Pleural Neoplasms,
pubmed-meshheading:20664962-Radiation Dosage,
pubmed-meshheading:20664962-Signal Transduction,
pubmed-meshheading:20664962-Time Factors,
pubmed-meshheading:20664962-Tumor Burden,
pubmed-meshheading:20664962-Xenograft Model Antitumor Assays
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| pubmed:year |
2010
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| pubmed:articleTitle |
Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model.
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| pubmed:affiliation |
Baylor College of Medicine, Michael E DeBakey Department of Surgery, Houston, TX 77030, USA. tmotomura@sbcglobal.net
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|