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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-8-15
|
| pubmed:abstractText |
Transfection of the hepatitis B virus (HBV) genome requires the cloning of tandem HBV sequences into a plasmid vector, which is usually screened for by restriction enzyme digestion of plasmid minipreparations from at least a dozen bacterial colonies. We describe a simple alternative screening method based on in situ hybridization of bacterial colonies with a [32P]-labelled synthetic oligonucleotide which spans the head-to-tail junction site of two tandem HBV molecules. The accurate detection by the oligoprobe is confirmed by enzymatic digestion.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0166-0934
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
109-14
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2066384-Base Sequence,
pubmed-meshheading:2066384-Cloning, Molecular,
pubmed-meshheading:2066384-Escherichia coli,
pubmed-meshheading:2066384-Hepatitis B virus,
pubmed-meshheading:2066384-Molecular Sequence Data,
pubmed-meshheading:2066384-Nucleic Acid Hybridization,
pubmed-meshheading:2066384-Oligonucleotide Probes,
pubmed-meshheading:2066384-Plasmids,
pubmed-meshheading:2066384-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2066384-Restriction Mapping,
pubmed-meshheading:2066384-Transfection
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Rapid screening for bacterial colonies harbouring tandem hepatitis B virus sequences by an oligonucleotide probe.
|
| pubmed:affiliation |
Unité de Recherche Sur Les Hépatites INSERM 271, Lyon, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|