Source:http://linkedlifedata.com/resource/pubmed/id/20663625
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2010-11-15
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pubmed:databankReference | |
pubmed:abstractText |
Bacterial strains from inoculated soybean field soil in Thailand were directly isolated using Bradyrhizobium japonicum selective medium (BJSM), on the basis of Zn(2+) and Co(2+) resistance of B. japonicum and B. elkanii. The isolates were classified into symbiotic and non-symbiotic groups by inoculation assays and Southern hybridization of nod and nif genes. In this study, a nearly full-length 16S rRNA gene sequence showed that the non-symbiotic isolates were more closely related to members of Rhodopseudomonas and to a number of uncultured bacterial clones than to members of Bradyrhizobium. Therefore, a polyphasic study was performed to determine the taxonomic positions of four representatives of the non-symbiotic isolates. Multilocus phylogenetic analysis of individual genes and a combination of the 16S rRNA and three housekeeping genes (atpD, recA and glnII) supported the placement of the non-symbiotic isolates in a different genus. The ability of heavy metal resistance in conjunction with phenotypic analyses, including cellular fatty acid content and biochemical characteristics, showed that the non-symbiotic isolates were differentiated from the other related genera in the family Bradyrhizobiaceae. Therefore, the non-symbiotic isolates represented a novel genus and species, for which the name Metalliresistens boonkerdii gen. nov., sp. nov. is proposed. The type strain is NS23 (= NBRC 106595(T)=BCC 40155(T)).
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Metals, Heavy,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1618-0984
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2010 Elsevier GmbH. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
374-82
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pubmed:dateRevised |
2011-4-25
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pubmed:meshHeading |
pubmed-meshheading:20663625-Bacterial Proteins,
pubmed-meshheading:20663625-Bacterial Typing Techniques,
pubmed-meshheading:20663625-Base Composition,
pubmed-meshheading:20663625-Base Sequence,
pubmed-meshheading:20663625-Bradyrhizobiaceae,
pubmed-meshheading:20663625-Cobalt,
pubmed-meshheading:20663625-Culture Media,
pubmed-meshheading:20663625-DNA, Bacterial,
pubmed-meshheading:20663625-Drug Resistance, Bacterial,
pubmed-meshheading:20663625-Fatty Acids,
pubmed-meshheading:20663625-Genes, rRNA,
pubmed-meshheading:20663625-Metals, Heavy,
pubmed-meshheading:20663625-Molecular Sequence Data,
pubmed-meshheading:20663625-Multilocus Sequence Typing,
pubmed-meshheading:20663625-Phenotype,
pubmed-meshheading:20663625-Phylogeny,
pubmed-meshheading:20663625-Polymerase Chain Reaction,
pubmed-meshheading:20663625-RNA, Ribosomal, 16S,
pubmed-meshheading:20663625-Sequence Analysis, DNA,
pubmed-meshheading:20663625-Soil Microbiology,
pubmed-meshheading:20663625-Soybeans,
pubmed-meshheading:20663625-Species Specificity,
pubmed-meshheading:20663625-Symbiosis,
pubmed-meshheading:20663625-Thailand,
pubmed-meshheading:20663625-Zinc
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pubmed:year |
2010
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pubmed:articleTitle |
Heavy metal tolerant Metalliresistens boonkerdii gen. nov., sp. nov., a new genus in the family Bradyrhizobiaceae isolated from soil in Thailand.
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pubmed:affiliation |
Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, Nakhonrachasima 30000, Thailand.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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