Source:http://linkedlifedata.com/resource/pubmed/id/20663492
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0023418,
umls-concept:C0023467,
umls-concept:C0026986,
umls-concept:C0030705,
umls-concept:C0035647,
umls-concept:C0039082,
umls-concept:C0265219,
umls-concept:C0332306,
umls-concept:C0439677,
umls-concept:C0681890,
umls-concept:C1511636,
umls-concept:C1879299,
umls-concept:C1948023,
umls-concept:C2698684
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| pubmed:issue |
1
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| pubmed:dateCreated |
2010-8-30
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| pubmed:abstractText |
Myeloid leukemic cells can differentiate into leukemia-derived dendritic cells (DC(leu)), presenting known/unknown leukemic-antigens. Induced anti-leukemic T-cell-responses are variable. To further elicit DC/DC(leu)-induced T-cell-response-patterns we performed (functional)flow-cytometry/fluorolysis-assays before/after mixed lymphocyte cultures (MLC) of matched (allogeneic) donor-T-cells (n=6), T-cells prepared at relapse after stem cell transplantation (n=4) or (autologous) patients'-T-cells (n=7) with blast-containing-mononuclear-cells ('MNC') or DC(leu)-containing DC ('DC'). Compared to 'MNC' 'DC' were better mediators of anti-leukaemic T-cell-activity, although not in every case effective. We could define cut-off proportions of mature DC, DC(leu), proliferating, CD4(+), CD8(+) and non-naive T-cells after 'MNC'- or 'DC'-stimulation, that were predictive for an anti-leukemic-activity of stimulated T-cells as well as a response to immunotherapy. Interestingly especially ratios >1 of CD4:CD8 or CD45RO:CD45RA T-cells were predictive for anti-leukemic function after DC-stimulation. In summary the composition and quality of DC and T-cells after a MLC-stimulating-phase is predictive for a successful ex-vivo and in-vivo anti-leukemic response, especially with respect to proportions of proliferating, CD4(+) and CD45RO(+) T-cells. Successful cytotoxicity and the development of a T-cell-memory after 'DC'-stimulation could be predictive for the clinical course of the disease and may pave the way to develop adoptive immunotherapy, especially for patients at relapse after SCT.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1090-2163
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| pubmed:author |
pubmed-author:AnsprengerChristianC,
pubmed-author:DreyssigJuliaJ,
pubmed-author:FreudenreichMarkusM,
pubmed-author:GrabruckerChristineC,
pubmed-author:KolbHans-JochemHJ,
pubmed-author:KremserAndreasA,
pubmed-author:KroellTanjaT,
pubmed-author:LiepertAnjaA,
pubmed-author:ReibkeRolandR,
pubmed-author:SchmetzerHelgaH,
pubmed-author:SchmidChristophC,
pubmed-author:SchweigerCorneliaC,
pubmed-author:TischerJohannaJ
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| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
265
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
23-30
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| pubmed:meshHeading |
pubmed-meshheading:20663492-Adaptive Immunity,
pubmed-meshheading:20663492-Adolescent,
pubmed-meshheading:20663492-Adult,
pubmed-meshheading:20663492-Aged,
pubmed-meshheading:20663492-Child,
pubmed-meshheading:20663492-Cytotoxicity, Immunologic,
pubmed-meshheading:20663492-Dendritic Cells,
pubmed-meshheading:20663492-Female,
pubmed-meshheading:20663492-Flow Cytometry,
pubmed-meshheading:20663492-Humans,
pubmed-meshheading:20663492-Leukemia, Myeloid, Acute,
pubmed-meshheading:20663492-Lymphocyte Activation,
pubmed-meshheading:20663492-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:20663492-Male,
pubmed-meshheading:20663492-Middle Aged,
pubmed-meshheading:20663492-Myelodysplastic Syndromes,
pubmed-meshheading:20663492-T-Lymphocytes,
pubmed-meshheading:20663492-Young Adult
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| pubmed:year |
2010
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| pubmed:articleTitle |
Quality of T-cells after stimulation with leukemia-derived dendritic cells (DC) from patients with acute myeloid leukemia (AML) or myeloid dysplastic syndrome (MDS) is predictive for their leukemia cytotoxic potential.
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| pubmed:affiliation |
University of Munich, Department for Haematopoietic Transplantations, Munich, Germany.
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| pubmed:publicationType |
Journal Article
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