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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
14
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pubmed:dateCreated |
1991-8-14
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pubmed:abstractText |
We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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|
pubmed:issn |
0021-9193
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
173
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pubmed:geneSymbol |
uidA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4540-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2066346-Bacteroides,
pubmed-meshheading:2066346-DNA Replication,
pubmed-meshheading:2066346-Escherichia coli,
pubmed-meshheading:2066346-Genes, Bacterial,
pubmed-meshheading:2066346-Genetic Vectors,
pubmed-meshheading:2066346-Glucuronidase,
pubmed-meshheading:2066346-Plasmids,
pubmed-meshheading:2066346-Promoter Regions, Genetic,
pubmed-meshheading:2066346-Restriction Mapping
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pubmed:year |
1991
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pubmed:articleTitle |
Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.
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pubmed:affiliation |
Department of Microbiology, University of Illinois, Urbana 61801.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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