Linked Life Data

20662595

Source:http://linkedlifedata.com/resource/pubmed/id/20662595

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
2010-11-24
pubmed:abstractText
Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) has been recognized as an appropriate technology for biomarker discovery. Nevertheless, whenever human blood serum is studied, its sensitivity is restricted due to the wide dynamic range of serum protein concentrations. In this context, sample pre-fractionation targeting the low abundant proteins may help to overcome these limitations. Here, we evaluate the combination of ProteoMiner™ pre-fractionation and SELDI based protein profiling. We introduce a simplified workflow and demonstrate the high sensitivity and reproducibility of this combined profiling approach. Our analyses show that this combination is suitable for large-scale serum proteome profiling studies yielding reliable and reproducible results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1744-4160
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
174-80
pubmed:meshHeading
pubmed:articleTitle
ProteoMiner™ and SELDI-TOF-MS: a robust and highly reproducible combination for biomarker discovery from whole blood serum.
pubmed:affiliation
Department of Clinical Biochemistry and Pathobiochemistry, Heinrich-Heine-University, Leibniz Centre for Diabetes Research, Düsseldorf, Germany. julia.froebel@med.uni-duesseldorf.de
pubmed:publicationType
Journal Article