Linked Life Data

20652765

Source:http://linkedlifedata.com/resource/pubmed/id/20652765

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pubmed-article:20652765pubmed:abstractTextThis study evaluated the feasibility of using commercially available immortalized cell lines in building an in vitro blood-brain barrier (BBB) co-culture model for preliminary drug development studies. Astrocytes-derived acellular extracellular matrix (aECM) was introduced in the co-culture model to provide a novel biomimetic basement membrane for the endothelial cells to form tight junctions. Trans-Endothelial Electrical Resistance (TEER) and solute mass transport studies quantitatively evaluated the tight junction formation. Immuno-fluorescence microscopy and Western blot analysis qualitatively verified the expression of occludin, one of the tight junction proteins on the samples. Experimental data from a total of 13 experiments conclusively showed that the novel BBB in vitro co-culture model with aECM (CO?+?aECM) is promising in terms of establishing tight junction formation represented by TEER values, transport profiles, and tight junction protein expression when compared with traditional co-culture (CO) model setup or the endothelial cells cultured alone (EC). In vitro colorimetric sulforhodamine B (SRB) assay also revealed that the "CO?+?aECM" samples resulted in less cell loss on the basal sides of the insert membranes than traditional co-culture models. Our novel approach using immortalized cell lines with the addition of aECM was proven to be a feasible and repeatable alternative to the traditional BBB in vitro modeling.lld:pubmed
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pubmed-article:20652765pubmed:authorpubmed-author:LiChen-ZhongC...lld:pubmed
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pubmed-article:20652765pubmed:articleTitleCo-culture based blood-brain barrier in vitro model, a tissue engineering approach using immortalized cell lines for drug transport study.lld:pubmed
pubmed-article:20652765pubmed:affiliationDepartment of Biomedical Engineering, Florida International University, Miami, 33174, USA.lld:pubmed
pubmed-article:20652765pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20652765pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed