Source:http://linkedlifedata.com/resource/pubmed/id/20652765
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-2-2
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| pubmed:abstractText |
This study evaluated the feasibility of using commercially available immortalized cell lines in building an in vitro blood-brain barrier (BBB) co-culture model for preliminary drug development studies. Astrocytes-derived acellular extracellular matrix (aECM) was introduced in the co-culture model to provide a novel biomimetic basement membrane for the endothelial cells to form tight junctions. Trans-Endothelial Electrical Resistance (TEER) and solute mass transport studies quantitatively evaluated the tight junction formation. Immuno-fluorescence microscopy and Western blot analysis qualitatively verified the expression of occludin, one of the tight junction proteins on the samples. Experimental data from a total of 13 experiments conclusively showed that the novel BBB in vitro co-culture model with aECM (CO?+?aECM) is promising in terms of establishing tight junction formation represented by TEER values, transport profiles, and tight junction protein expression when compared with traditional co-culture (CO) model setup or the endothelial cells cultured alone (EC). In vitro colorimetric sulforhodamine B (SRB) assay also revealed that the "CO?+?aECM" samples resulted in less cell loss on the basal sides of the insert membranes than traditional co-culture models. Our novel approach using immortalized cell lines with the addition of aECM was proven to be a feasible and repeatable alternative to the traditional BBB in vitro modeling.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fluorexon,
http://linkedlifedata.com/resource/pubmed/chemical/occludin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1559-0291
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
163
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
278-95
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| pubmed:meshHeading |
pubmed-meshheading:20652765-Animals,
pubmed-meshheading:20652765-Astrocytes,
pubmed-meshheading:20652765-Biological Transport,
pubmed-meshheading:20652765-Blood-Brain Barrier,
pubmed-meshheading:20652765-Blotting, Western,
pubmed-meshheading:20652765-Caffeine,
pubmed-meshheading:20652765-Cell Line,
pubmed-meshheading:20652765-Coculture Techniques,
pubmed-meshheading:20652765-Electric Impedance,
pubmed-meshheading:20652765-Endothelial Cells,
pubmed-meshheading:20652765-Extracellular Matrix,
pubmed-meshheading:20652765-Fluoresceins,
pubmed-meshheading:20652765-Gene Expression,
pubmed-meshheading:20652765-Membrane Proteins,
pubmed-meshheading:20652765-Mice,
pubmed-meshheading:20652765-Models, Biological,
pubmed-meshheading:20652765-Rats,
pubmed-meshheading:20652765-Reproducibility of Results,
pubmed-meshheading:20652765-Tight Junctions,
pubmed-meshheading:20652765-Tissue Engineering
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| pubmed:year |
2011
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| pubmed:articleTitle |
Co-culture based blood-brain barrier in vitro model, a tissue engineering approach using immortalized cell lines for drug transport study.
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| pubmed:affiliation |
Department of Biomedical Engineering, Florida International University, Miami, 33174, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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