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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1991-8-15
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pubmed:abstractText |
The EGR-1 gene is an immediate early response gene encoding a zinc finger DNA-binding protein. The present studies have examined the regulation of EGR-1 gene expression in human U-937 monocytic leukemia cells treated with granulocyte-macrophage colony-stimulating factor (GM-CSF). The results demonstrate that GM-CSF rapidly and transiently increases EGR-1 gene expression in U-937 cells. Similar findings were obtained in GM-CSF-treated human monocytes. We also show that the regulation of EGR-1 expression by GM-CSF is a pertussis toxin-sensitive event. The results of nuclear run-on assays further demonstrate that the EGR-1 gene is constitutively transcribed in untreated U-937 cells and that GM-CSF has little effect on this rate of transcription. Inhibition of protein synthesis with cycloheximide also had no detectable effect on EGR-1 gene transcription but was associated with superinduction of EGR-1 mRNA levels in GM-CSF-treated cells. Moreover, the half-life of GM-CSF-induced EGR-1 transcripts was prolonged from 33 to 70 min following inhibition of protein synthesis. Taken together, the results indicate that GM-CSF activates signaling pathways which regulate EGR-1 gene expression through a pertussis toxin-sensitive G protein and that these events increase EGR-1 mRNA levels by a posttranscriptional mechanism. This GM-CSF-dependent regulation of EGR-1 expression may provide a mechanism for transducing signals to the nucleus that are involved in the control of gene transcription.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
1044-9523
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
2
|
pubmed:geneSymbol |
ERG-1
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
273-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2064996-Cell Line, Transformed,
pubmed-meshheading:2064996-Gene Expression Regulation, Leukemic,
pubmed-meshheading:2064996-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2064996-Humans,
pubmed-meshheading:2064996-Leukemia, Myeloid,
pubmed-meshheading:2064996-Monocytes,
pubmed-meshheading:2064996-Pertussis Toxin,
pubmed-meshheading:2064996-RNA Processing, Post-Transcriptional,
pubmed-meshheading:2064996-Transcription, Genetic,
pubmed-meshheading:2064996-Virulence Factors, Bordetella,
pubmed-meshheading:2064996-Zinc Fingers
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pubmed:year |
1991
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pubmed:articleTitle |
Posttranscriptional regulation of the zinc finger-encoding EGR-1 gene by granulocyte-macrophage colony-stimulating factor in human U-937 monocytic leukemia cells: involvement of a pertussis toxin-sensitive G protein.
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pubmed:affiliation |
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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