20642492

Source:http://linkedlifedata.com/resource/pubmed/id/20642492

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0017366, umls-concept:C0021201, umls-concept:C0042210, umls-concept:C0080194, umls-concept:C0206538, umls-concept:C0370215, umls-concept:C1519068, umls-concept:C1519249, umls-concept:C1520022
pubmed:issue	5
pubmed:dateCreated	2010-11-5
pubmed:abstractText	Peste des petits ruminants (PPR) is an acute, highly contagious, notifiable and economically important transboundary viral disease of sheep and goats. In this study, sequence and phylogenetic analyses of structural protein genes, namely the nucleocapsid (N), the matrix (M), the fusion (F) and the haemagglutinin (H) coding sequences of virulent and vaccine strains of PPR virus (PPRV), were undertaken to determine the genetic variations between field isolates and vaccine strains. The open reading frame (ORF) of these genes of the isolates/strains was amplified by RT-PCR, cloned and sequenced. The ORF of N, M, F and H genes was 1578, 1008, 1641 and 1830 nucleotides (nt) in length and encodes polypeptides of 525, 335, 546 and 609 amino acids (aa), respectively, as reported earlier. Comparative sequence analyses of these four genes of isolates/strains were carried out with published sequences. It revealed an identity of 97.7-100% and 97.7-99.8% among the Asian lineage IV and 89.6-98.7% and 89.8-98.9% with other lineages of PPRV at nt and aa levels, respectively. The phylogenetic analyses of these isolates based on the aa sequences showed that all the viruses belonged to lineage IV along with other Asian isolates. This is in agreement with earlier observations that only PPRV lineage IV is in circulation in India since the disease was first reported. Further, sequence analysis of the thermostable/thermo-adapted vaccine strains showed no significant changes in the functional or structural surface protein-coding gene sequences. It is important to monitor the circulation of the PPRV in susceptible animals by H gene-based sequence comparisons in addition to the F gene- and N gene-based approaches to identify the distribution and spread of virus in the regular outbreaks that occur in endemic countries like India.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101319538
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	1865-1682
pubmed:author	pubmed-author:BalamuruganVV, pubmed-author:BhanotVV, pubmed-author:BhanuprakashVV, pubmed-author:RiyeshTT, pubmed-author:SenAA, pubmed-author:SinghR KRK, pubmed-author:VenkatesanGG, pubmed-author:YadavVV
pubmed:copyrightInfo	© 2010 Blackwell Verlag GmbH.
pubmed:issnType	Electronic
pubmed:volume	57
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	352-64
pubmed:meshHeading	pubmed-meshheading:20642492-Animals, pubmed-meshheading:20642492-India, pubmed-meshheading:20642492-Peste-des-Petits-Ruminants, pubmed-meshheading:20642492-Peste-des-petits-ruminants virus
pubmed:year	2010
pubmed:articleTitle	Sequence and phylogenetic analyses of the structural genes of virulent isolates and vaccine strains of peste des petits ruminants virus from India.
pubmed:affiliation	Division of Virology, Indian Veterinary Research Institute, Nainital District, Uttarakhand, India. balavirol@gmail.com
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't