Linked Life Data

20641014

Source:http://linkedlifedata.com/resource/pubmed/id/20641014

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-9-8
pubmed:databankReference
pubmed:abstractText
In this work, we developed molecular tools used in standard laboratory yeast strains, such as the cre-loxP system, so that they can be used with natural and industrial prototrophic yeast species. We constructed a new generation of dominant cassettes, with mutated loxP sites (loxLE and lox2272) and selectable drug markers, to create heterothallic strains and auxotrophic mutants without incurring in the risk of generating chromosomal rearrangements. We have shown that our newly developed loxLE-hphNT1-loxRE and lox2272-natNT2-lox2272 gene-disruption cassettes can be present in the yeast genome together with the widely used loxP-marker gene-loxP cassettes without any recombination between the lox sequences. Moreover, we also developed a new phleomycin-resistant Cre-expressing vector (to excise multiple markers simultaneously) and two new standard loxP deletion cassettes containing hygromicin B and cloNAT as selecatable markers. To validate these cassettes, we created heterothallic auxotrophic S. cerevisiae strains, without the risk of incurring gross chromosomal rearrangements, and we showed an example of a fitness study of intraspecific hybrids deriving from parents with different adaptations to carbon-limited resources.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1097-0061
pubmed:author
pubmed:copyrightInfo
Copyright 2010 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
765-75
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
New generation of loxP-mutated deletion cassettes for the genetic manipulation of yeast natural isolates.
pubmed:affiliation
Faculty of Life Sciences, Manchester University, Manchester, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't