Linked Life Data

20638501

Source:http://linkedlifedata.com/resource/pubmed/id/20638501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-7-19
pubmed:abstractText
The principal objective of this study was to develop an appropriate, sensitive, and selective method for the simultaneous quantitative determination of phoxim and its photo-transformation product, O,O-diethyl alpha-cyanobenzylideneamino-thiophosphonate (DCTP) in both chicken and quail eggs. Eggs (1 g) were blended with anhydrous magnesium sulfate (1 g) for sample pretreatment and extracted with acetonitrile. The extracts were then further purified with SPE silica gel tubes deactivated with trimethylamine. Residues were analyzed via a reversed phase-liquid chromatography-tandem mass spectrometry (RP-LC-MS/MS) in positive-ion electrospray ionization (ESI) mode. Tebufenozide was utilized as an internal standard for the quantification of phoxim and its metabolite residues. The identification and quantification of analytes were based on ion transitions monitored by multiple reaction monitoring (MRM). LC-MS/MS analysis was performed from 0.02 to 1 mg kg(-1) and correlation coefficients (r(2)) ranging from 0.998 to 0.999 were obtained for both analytes in blank egg extracts. The relative standard deviations (RSDs) of intra- and inter-day variations ranged from 2.1% to 6.7% and from 2.8% to 6.4% for phoxim and DCTP in chicken and quail eggs. At all levels of fortification (0.02, 0.05, and 0.125 mg kg(-1)), the recoveries fell within a range of 81.3% to 93.6% for phoxim and 83.3% to 90.1% for DCTP. The matrix effect was <2%, due to the partial dilution of the sample. Decision limits (CCalpha) and detection capabilities (CCbeta) were in the range of 0.0005-0.0044 and 0.0054-0.0224 mg kg(-1), respectively. The method was evaluated further by analyzing real samples purchased from markets. All chicken and quail egg samples were free from residues of the target compounds.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1873-4324
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
674
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
64-70
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Simultaneous determination of phoxim and its photo-transformation metabolite residues in eggs using liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
pubmed:affiliation
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't