Source:http://linkedlifedata.com/resource/pubmed/id/20637880
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2011-2-14
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| pubmed:abstractText |
Donor lymphocyte infusions (DLIs) are used for adoptive immunotherapy to prevent or treat relapse and infectious complications after allogeneic hematopoietic stem cell transplantation (HSCT). Unmanipulated DLIs are associated with a risk of graft-versus-host disease (GVHD), probably related to CD8(+) T cell activity. We investigated an automated clinical-scale human-CD4(+)-cell purification method to deplete CD8(+) cells. Twenty-four stem cell recipients received a total of 24 leukapheresis products being enriched for CD4(+) cells using magnetic associated cell sorting (MACS) with an automated device (CliniMACS(®)) before DLIs. MACS resulted in a mean CD4(+) cell count of 16 × 10(6)/kg bw corresponding to 3.4-fold CD4(+) cell enrichment. Mean yield and purity of CD45(+)CD3(+)CD4(+)CD14(-)7AAD(-) were 74% ± 23% and 82% ± 11%, respectively. Median initial dose of DLIs was 1.1 × 10(6) CD4(+)/kg. During a median follow-up of 25 months, 7 (30%) patients experienced GVHD (acute II-IV: n = 4, 17%; acute III-IV: n = 2, 8%; chronic limited: n = 2, 8%; chronic extensive: n = 1, 4%). Thirteen of 21 further evaluable patients (62%) showed measurable clinical response, 2 patients with therapy refractory infectious complications (HSV) showed remarkable immunologic improvement. Automated enrichment of CD4(+) by magnetic cell sorting provides an efficient and rapid method for processing donor lymphocytes. Additional studies should further investigate this approach in terms of efficacy and the risk of GVHD.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1523-6536
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| pubmed:author |
pubmed-author:AdjalléRaissaR,
pubmed-author:AmtsfeldGittaG,
pubmed-author:AyukFrancisF,
pubmed-author:BacherUlrikeU,
pubmed-author:FehseBorisB,
pubmed-author:KlyuchnikovEvgenyE,
pubmed-author:KrögerNicolausN,
pubmed-author:LioznovMichaelM,
pubmed-author:MerleEdeltrautE,
pubmed-author:ReckhausMarie-LuiseML,
pubmed-author:SlesarchukOlgaO,
pubmed-author:SputtekAndreasA,
pubmed-author:StübigThomasT,
pubmed-author:WolschkeChristineC,
pubmed-author:ZanderAxelA
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| pubmed:copyrightInfo |
Copyright © 2011 American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
374-83
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| pubmed:meshHeading |
pubmed-meshheading:20637880-Adult,
pubmed-meshheading:20637880-Aged,
pubmed-meshheading:20637880-CD4-Positive T-Lymphocytes,
pubmed-meshheading:20637880-Cohort Studies,
pubmed-meshheading:20637880-Female,
pubmed-meshheading:20637880-Follow-Up Studies,
pubmed-meshheading:20637880-Graft vs Host Disease,
pubmed-meshheading:20637880-Hematologic Neoplasms,
pubmed-meshheading:20637880-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:20637880-Humans,
pubmed-meshheading:20637880-Immunomagnetic Separation,
pubmed-meshheading:20637880-Immunotherapy, Adoptive,
pubmed-meshheading:20637880-Male,
pubmed-meshheading:20637880-Middle Aged,
pubmed-meshheading:20637880-Multiple Myeloma,
pubmed-meshheading:20637880-Opportunistic Infections,
pubmed-meshheading:20637880-Recurrence,
pubmed-meshheading:20637880-Retrospective Studies,
pubmed-meshheading:20637880-Survival Analysis,
pubmed-meshheading:20637880-Young Adult
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| pubmed:year |
2011
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| pubmed:articleTitle |
Purification of CD4+ T cells for adoptive immunotherapy after allogeneic hematopoietic stem cell transplantation.
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| pubmed:affiliation |
Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
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| pubmed:publicationType |
Journal Article
|