Linked Life Data

20637660

Source:http://linkedlifedata.com/resource/pubmed/id/20637660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-7-20
pubmed:abstractText
Transcriptional regulation of the Nos2 gene encoding inducible nitric oxide synthase (iNOS) requires type I interferon (IFN-I) signaling and additional signals emanating from pattern recognition receptors. Here we showed sequential and cooperative contributions of the transcription factors ISGF3 (a complex containing STAT1, STAT2, and IRF9 subunits) and NF-kappaB to the transcriptional induction of the Nos2 gene in macrophages infected with the intracellular bacterial pathogen Listeria monocytogenes. NF-kappaB preceded ISGF3 at the Nos2 promoter and generated a transcriptional memory effect by depositing basal transcription factor TFIIH with the associated CDK7 kinase for serine 5 phosphorylation of the RNA polymerase II (pol II) carboxyterminal domain (CTD). Subsequent to TFIIH deposition by NF-kappaB, ISGF3 attracted the pol II enzyme and phosphorylation at CTD S5 occurred. Thus, STATs and NF-kappaB cooperate through pol II promoter recruitment and the phosphorylation of its CTD, respectively, as a prerequisite for productive elongation of iNOS mRNA.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1097-4180
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-34
pubmed:dateRevised
2011-9-29
pubmed:meshHeading
pubmed-meshheading:20637660-Animals, pubmed-meshheading:20637660-Cells, Cultured, pubmed-meshheading:20637660-Cyclin-Dependent Kinases, pubmed-meshheading:20637660-Gene Expression Regulation, Enzymologic, pubmed-meshheading:20637660-Interferon Type I, pubmed-meshheading:20637660-Interferon-Stimulated Gene Factor 3, pubmed-meshheading:20637660-Listeria monocytogenes, pubmed-meshheading:20637660-Listeriosis, pubmed-meshheading:20637660-Macrophages, pubmed-meshheading:20637660-Mice, pubmed-meshheading:20637660-Mice, Inbred C57BL, pubmed-meshheading:20637660-Mice, Knockout, pubmed-meshheading:20637660-NF-kappa B, pubmed-meshheading:20637660-Nitric Oxide Synthase Type II, pubmed-meshheading:20637660-Protein Binding, pubmed-meshheading:20637660-RNA Polymerase II, pubmed-meshheading:20637660-STAT Transcription Factors, pubmed-meshheading:20637660-Transcription Factor TFIIH
pubmed:year
2010
pubmed:articleTitle
Nonconventional initiation complex assembly by STAT and NF-kappaB transcription factors regulates nitric oxide synthase expression.
pubmed:affiliation
Max F. Perutz Laboratories, Department of Genetics, Microbiology and Immunobiology, University of Vienna, Dr. Bohr-Gasse 9/4, A1030 Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't