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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-10-11
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pubmed:abstractText |
Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound, doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1873-4863
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-81
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pubmed:meshHeading |
pubmed-meshheading:20633583-Biological Markers,
pubmed-meshheading:20633583-Biosensing Techniques,
pubmed-meshheading:20633583-Cell Count,
pubmed-meshheading:20633583-Cell Line,
pubmed-meshheading:20633583-Dose-Response Relationship, Drug,
pubmed-meshheading:20633583-Doxorubicin,
pubmed-meshheading:20633583-Embryonic Stem Cells,
pubmed-meshheading:20633583-Fatty Acid-Binding Proteins,
pubmed-meshheading:20633583-Gene Expression,
pubmed-meshheading:20633583-Heart Diseases,
pubmed-meshheading:20633583-Humans,
pubmed-meshheading:20633583-Immunohistochemistry,
pubmed-meshheading:20633583-Myocytes, Cardiac,
pubmed-meshheading:20633583-Polymerase Chain Reaction,
pubmed-meshheading:20633583-Surface Plasmon Resonance,
pubmed-meshheading:20633583-Toxicity Tests,
pubmed-meshheading:20633583-Troponin T
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pubmed:year |
2010
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pubmed:articleTitle |
Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.
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pubmed:affiliation |
Division of Biotechnology/IFM, Linköping University, 581 83 Linköping, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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