Linked Life Data

20633124

Source:http://linkedlifedata.com/resource/pubmed/id/20633124

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-7-16
pubmed:abstractText
Histone acetylation/deacetylation is a central mechanism for regulating transcription through chromatin remodeling. The brain-derived neurotrophic factor gene (Bdnf) is regulated in part through chromatin remodeling. An inhibitor of histone deacetylase (HDAC) activity, Trichostatin A (TSA), has differential effects on two activation dependent regions of the Bdnf gene physically linked to transcription sites for exons 1 and 4. We determined that TSA treatment of cultures of hippocampal neurons produced a stronger response at promoter 1. Transcriptional activation of promoter 1 correlated with increased occupancy of the promoter by acetylated histones (H3AcK9/K14). TSA treatment also produced a time-dependent increase in the level of H3AcK9 and H3AcK14 protein and Hdac1 mRNA levels and HDAC1 protein levels. Taken together, these findings suggest that inhibition of HDAC activity by TSA activates Bdnf transcription and a compensatory change in HDAC1 expression in neurons. This response may reflect a genome-wide change in gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1749-6632
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
1199
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
186-93
pubmed:meshHeading
pubmed-meshheading:20633124-Animals, pubmed-meshheading:20633124-Base Sequence, pubmed-meshheading:20633124-Blotting, Western, pubmed-meshheading:20633124-Brain-Derived Neurotrophic Factor, pubmed-meshheading:20633124-Cells, Cultured, pubmed-meshheading:20633124-Chromatin Immunoprecipitation, pubmed-meshheading:20633124-DNA Primers, pubmed-meshheading:20633124-Epigenesis, Genetic, pubmed-meshheading:20633124-Hippocampus, pubmed-meshheading:20633124-Histone Deacetylase Inhibitors, pubmed-meshheading:20633124-Hydroxamic Acids, pubmed-meshheading:20633124-Neurons, pubmed-meshheading:20633124-Promoter Regions, Genetic, pubmed-meshheading:20633124-Rats, pubmed-meshheading:20633124-Rats, Sprague-Dawley, pubmed-meshheading:20633124-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20633124-Transcriptional Activation
pubmed:year
2010
pubmed:articleTitle
Effects of histone deacetylase inhibitor Trichostatin A on epigenetic changes and transcriptional activation of Bdnf promoter 1 by rat hippocampal neurons.
pubmed:affiliation
Section on Cancer Genetics, Genetics Branch, National Cancer Institute, NIH, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.