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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2010-10-29
pubmed:abstractText
The molecular basis of lymphangiogenesis remains incompletely characterized. Here, we document a novel role for the PDZ domain-containing scaffold protein synectin in lymphangiogenesis using genetic studies in zebrafish and tadpoles. In zebrafish, the thoracic duct arises from parachordal lymphangioblast cells, which in turn derive from secondary lymphangiogenic sprouts from the posterior cardinal vein. Morpholino knockdown of synectin in zebrafish impaired formation of the thoracic duct, due to selective defects in lymphangiogenic but not angiogenic sprouting. Synectin genetically interacted with Vegfr3 and neuropilin-2a in regulating lymphangiogenesis. Silencing of synectin in tadpoles caused lymphatic defects due to an underdevelopment and impaired migration of Prox-1(+) lymphatic endothelial cells. Molecular analysis further revealed that synectin regulated Sox18-induced expression of Prox-1 and vascular endothelial growth factor C-induced migration of lymphatic endothelial cells in vitro. These findings reveal a novel role for synectin in lymphatic development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1528-0020
pubmed:author
pubmed:issnType
Electronic
pubmed:day
28
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3356-66
pubmed:meshHeading
pubmed-meshheading:20631376-Animals, pubmed-meshheading:20631376-Carrier Proteins, pubmed-meshheading:20631376-Cell Line, pubmed-meshheading:20631376-Gene Expression Regulation, Developmental, pubmed-meshheading:20631376-Gene Silencing, pubmed-meshheading:20631376-Humans, pubmed-meshheading:20631376-Larva, pubmed-meshheading:20631376-Lymphangiogenesis, pubmed-meshheading:20631376-Lymphatic Vessels, pubmed-meshheading:20631376-Neovascularization, Physiologic, pubmed-meshheading:20631376-Neuropilin-2, pubmed-meshheading:20631376-Thoracic Duct, pubmed-meshheading:20631376-Vascular Endothelial Growth Factor C, pubmed-meshheading:20631376-Vascular Endothelial Growth Factor Receptor-3, pubmed-meshheading:20631376-Xenopus Proteins, pubmed-meshheading:20631376-Xenopus laevis, pubmed-meshheading:20631376-Zebrafish, pubmed-meshheading:20631376-Zebrafish Proteins
pubmed:year
2010
pubmed:articleTitle
Role of synectin in lymphatic development in zebrafish and frogs.
pubmed:affiliation
Vesalius Research Center, Vlaams Iustituut voor Biotechnologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49, Leuven, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't