Source:http://linkedlifedata.com/resource/pubmed/id/20623123
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2010-11-5
|
| pubmed:abstractText |
To identify a new diagnostic marker for the immune pathophysiology of aplastic anemia (AA), we screened sera of immune-mediated AA patients for the presence of antibodies (Abs) specific to proteins derived from a leukemia cell line UT-7 using two-dimensional electrophoresis followed by immunoblotting. The target proteins were identified by peptide mass fingerprinting. Heterogeneous nuclear ribonucleoprotein (hnRNP) K was identified as a novel autoantigen. An enzyme-linked immunosorbent assay revealed high titers of anti-hnRNP K Abs in 85 (31%) of 273 patients with AA. Sixty-four patients received antithymocyte globulin and cyclosporine after undergoing screening for anti-hnRNP K Ab, anti-DRS-1 Ab, anti-moesin Ab, and paroxysmal nocturnal hemoglobinuria (PNH)-type cells. Twenty (87%) of 23 patients with the presence of anti-hnRNP K Abs responded to the immunosuppressive therapy (IST), while 19 (46%) of 41 patients without the presence of anti-hnRNP K Abs responded. A multivariate analysis showed only PNH-type cells and anti-hnRNP K Abs to be significant factors for the prediction of a good response to IST. The detection of anti-hnRNP K Abs as well as PNH-type cells may therefore be useful for diagnosing the immune pathophysiology of AA.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1432-0584
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
89
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1255-63
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| pubmed:meshHeading |
pubmed-meshheading:20623123-Adolescent,
pubmed-meshheading:20623123-Adult,
pubmed-meshheading:20623123-Aged,
pubmed-meshheading:20623123-Aged, 80 and over,
pubmed-meshheading:20623123-Anemia, Aplastic,
pubmed-meshheading:20623123-Autoantibodies,
pubmed-meshheading:20623123-Biological Markers,
pubmed-meshheading:20623123-Blotting, Western,
pubmed-meshheading:20623123-Cell Line, Tumor,
pubmed-meshheading:20623123-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:20623123-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20623123-Female,
pubmed-meshheading:20623123-HL-60 Cells,
pubmed-meshheading:20623123-Heterogeneous-Nuclear Ribonucleoprotein K,
pubmed-meshheading:20623123-Humans,
pubmed-meshheading:20623123-K562 Cells,
pubmed-meshheading:20623123-Leukocytes, Mononuclear,
pubmed-meshheading:20623123-Male,
pubmed-meshheading:20623123-Middle Aged,
pubmed-meshheading:20623123-Young Adult
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Autoantibodies specific to hnRNP K: a new diagnostic marker for immune pathophysiology in aplastic anemia.
|
| pubmed:affiliation |
Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science, 13-1 Takaramachi, Kanazawa, 920-8641, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|