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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2010-8-4
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pubmed:abstractText |
Binding of the third variable region (V3) of the HIV-1 envelope glycoprotein gp120 to the cell-surface coreceptors CCR5 or CXCR4 during viral entry suggests that there are conserved structural elements in this sequence-variable region. These conserved elements could serve as epitopes to be targeted by a vaccine against HIV-1. Here we perform a systematic structural analysis of representative human anti-V3 monoclonal antibodies in complex with V3 peptides, revealing that the crown of V3 has four conserved structural elements: an arch, a band, a hydrophobic core and the peptide backbone. These are either unaffected by or are subject to minimal sequence variation. As these regions are targeted by cross-clade neutralizing human antibodies, they provide a blueprint for the design of vaccine immunogens that could elicit broadly cross-reactive protective antibodies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1545-9985
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
955-61
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:20622876-Amino Acid Sequence,
pubmed-meshheading:20622876-Antibodies, Monoclonal,
pubmed-meshheading:20622876-Antibody Specificity,
pubmed-meshheading:20622876-Conserved Sequence,
pubmed-meshheading:20622876-Crystallography, X-Ray,
pubmed-meshheading:20622876-Epitopes,
pubmed-meshheading:20622876-HIV Envelope Protein gp120,
pubmed-meshheading:20622876-Humans,
pubmed-meshheading:20622876-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:20622876-Immunoglobulin Fragments,
pubmed-meshheading:20622876-Models, Molecular,
pubmed-meshheading:20622876-Molecular Sequence Data,
pubmed-meshheading:20622876-Peptides,
pubmed-meshheading:20622876-Protein Structure, Secondary,
pubmed-meshheading:20622876-Protein Structure, Tertiary,
pubmed-meshheading:20622876-Sequence Alignment,
pubmed-meshheading:20622876-Species Specificity
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pubmed:year |
2010
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pubmed:articleTitle |
Conserved structural elements in the V3 crown of HIV-1 gp120.
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pubmed:affiliation |
Department of Biochemistry, New York University School of Medicine, New York, New York, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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