Source:http://linkedlifedata.com/resource/pubmed/id/20616361
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-8-31
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| pubmed:abstractText |
Determination of NPM1 mutation status has become essential for the molecular classification of acute myeloid leukemias (AML). Methods with high clinical sensitivity and specificity adapted to the molecular laboratory workflow are required for the diagnosis, prognosis, and monitoring of AML with normal karyotype. We report here the development and evaluation of a novel, streamlined, RNA-based assay for the rapid multiplex detection of common NPM1 mutations in a 96-well assay format. Using synthetic transcripts and total RNA from leukemic cell lines, we show that the assay can specifically detect NPM1 wild-type and mutants A, B, D, or J transcripts in the same reaction. Dilution experiments indicate an assay dynamic range >4 log units with an analytical sensitivity of approximately 0.01%. Evaluation of 69 clinical specimens at initial diagnosis resulted in 100% agreement with reference methods. Of patients with AML with normal karyotype, 53% carried one of four different mutations. The assay was also combined with other laboratory-developed tests to simultaneously detect NPM1 mutant transcripts and fusion transcripts resulting from t(8;21) or inv(16) in a single reaction well. Overall, these results show that the assay is a versatile and specific tool for the screening of NPM1 mutations in patients with AML. Its high analytical sensitivity further suggests potential utility for the monitoring of residual disease in AML with normal karyotype.
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| pubmed:grant | |
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-10211837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-11051553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-12080348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-15659725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-15994285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16046528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16051734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16076867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16109776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16244291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-16455950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-17008539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-17041639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-18450602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-19055671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-19100523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-19429869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-19770764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20616361-2914325
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
|
| pubmed:issn |
1943-7811
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
12
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
629-35
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| pubmed:dateRevised |
2011-9-13
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| pubmed:meshHeading | |
| pubmed:year |
2010
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| pubmed:articleTitle |
Performance and clinical evaluation of a sensitive multiplex assay for the rapid detection of common NPM1 mutations.
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| pubmed:affiliation |
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
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| pubmed:publicationType |
Journal Article,
Evaluation Studies,
Research Support, N.I.H., Extramural
|