Linked Life Data

20615200

Source:http://linkedlifedata.com/resource/pubmed/id/20615200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-9-20
pubmed:abstractText
Clinical application of viral vectors is often hampered by the lack of selectivity of viral particles for the targeted tissue. This drawback decreases the efficiency of gene delivery and raises safety concerns. We successfully established a novel in vitro evolution protocol to engineer adeno-associated virus vectors with increased selectivity for designated target cells. Subjecting a peptide-display library of AAV capsids to negative selection cycles on human primary fibroblasts and to positive selection cycles on a human melanoma cell line, we isolated several variants with up to 3.7-fold increased specificity for malignant cells in comparison to fibroblasts and other cell types. These mutants can be used to achieve high levels of gene transfer to target cells reducing undesired transduction of neighbouring tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1875-5402
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-12
pubmed:dateRevised
2011-9-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A novel directed evolution method to enhance cell-type specificity of adeno-associated virus vectors.
pubmed:affiliation
Clinic I for Internal Medicine, University Hospital of Cologne, Kerpener Str. 62, 50937 Cologne, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't