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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2010-8-19
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pubmed:abstractText |
In this study, Equus caballus major histocompatibility complex class I (MHC-I) was identified as a cellular entry receptor for the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This novel EHV-1 receptor was discovered using a cDNA library from equine macrophages. cDNAs from this EHV-1-susceptible cell type were inserted into EHV-1-resistant B78H1 murine melanoma cells, these cells were infected with an EHV-1 lacZ reporter virus, and cells that supported virus infection were identified by X-Gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining. Positive cells were subjected to several rounds of purification to obtain homogeneous cell populations that were shown to be uniformly infected with EHV-1. cDNAs from these cell populations were amplified by PCR and then sequenced. The sequence data revealed that the EHV-1-susceptible cells had acquired an E. caballus MHC-I cDNA. Cell surface expression of this receptor was verified by confocal immunofluorescence microscopy. The MHC-I cDNA was cloned into a mammalian expression vector, and stable B78H1 cell lines were generated that express this receptor. These cell lines were susceptible to EHV-1 infection while the parental B78H1 cells remained resistant to infection. In addition, EHV-1 infection of the B78H1 MHC-I-expressing cell lines was inhibited in a dose-dependent manner by an anti-MHC-I antibody.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-10082388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-10355775,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-17670830,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-8612235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-8625414,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-8945469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20610718-9672642
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1098-5514
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9027-34
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pubmed:dateRevised |
2011-7-25
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pubmed:meshHeading |
pubmed-meshheading:20610718-Animals,
pubmed-meshheading:20610718-Cell Line,
pubmed-meshheading:20610718-Cell Membrane,
pubmed-meshheading:20610718-Gene Expression,
pubmed-meshheading:20610718-Gene Library,
pubmed-meshheading:20610718-Genes, Reporter,
pubmed-meshheading:20610718-Herpesvirus 1, Equid,
pubmed-meshheading:20610718-Histocompatibility Antigens Class I,
pubmed-meshheading:20610718-Horses,
pubmed-meshheading:20610718-Macrophages,
pubmed-meshheading:20610718-Mice,
pubmed-meshheading:20610718-Microscopy, Confocal,
pubmed-meshheading:20610718-Microscopy, Fluorescence,
pubmed-meshheading:20610718-Receptors, Virus,
pubmed-meshheading:20610718-Sequence Analysis, DNA,
pubmed-meshheading:20610718-Staining and Labeling,
pubmed-meshheading:20610718-Transfection,
pubmed-meshheading:20610718-Virus Internalization,
pubmed-meshheading:20610718-beta-Galactosidase
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pubmed:year |
2010
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pubmed:articleTitle |
Equus caballus major histocompatibility complex class I is an entry receptor for equine herpesvirus type 1.
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pubmed:affiliation |
Department of Biology and Marine Biology, University of North Carolina Wilmington, 601 S. College Rd., Wilmington, NC 28403, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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