Linked Life Data

20609919

Source:http://linkedlifedata.com/resource/pubmed/id/20609919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-7-8
pubmed:abstractText
Increasing evidence supports that reactive oxygen species (ROS) generated from mitochondria in vasculature significantly contribute to human disease. The mitochondrial antioxidant systems, particularly the redox protein thioredoxin-2 (Trx2), provide a primary line of defense against cellular ROS. Using endothelial cell culture and endothelial cell-specific transgenesis of Trx2 gene in mice, we demonstrate the critical roles of Trx2 in regulating endothelium functions. Here, we describe the methods related to generation and characterization of the Trx2 transgenic mice, and the in vivo functional assays associated with Trx2 activities. These methods could be applied to functional analyses for other redox genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1557-7988
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
474
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-24
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Thioredoxin and redox signaling in vasculature-studies using Trx2 endothelium-specific transgenic mice.
pubmed:affiliation
Interdepartmental Program in Vascular Biology and Therapeutics, Yale University School of Medicine, New Haven, Connecticut, USA.
pubmed:publicationType
Journal Article