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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1991-8-7
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pubmed:abstractText |
The objective of this study was to test the hypothesis that the extracellular oxidation of glutathione (GSH) may represent an important mechanism to limit hepatic ischemia/reperfusion injury in male Fischer rats in vivo. Basal plasma levels of glutathione disulfide (GSSG: 1.5 +/- 0.2 microM GSH-equivalents), glutathione (GSH: 6.2 +/- 0.4 microM) and alanine aminotransferase activities (ALT: 12 +/- 2 U/l) were significantly increased during the 1 h reperfusion period following 1 h of partial hepatic no-flow ischemia (GSSG: 19.7 +/- 2.2 microM; GSH 36.9 +/- 7.4 microM; ALT: 2260 +/- 355 U/l). Pretreatment with 1,3-bis-(2-chloroethyl)-1-nitrosourea (40 mg BCNU/kg), which inhibited glutathione reductase activity in the liver by 60%, did not affect any of these parameters. Biliary GSSG and GSH efflux rates were reduced and the GSSG-to-GSH ratio was not altered in controls and BCNU-treated rats at any time during ischemia and reperfusion. A 90% depletion of the hepatic glutathione content by phorone treatment (300 mg/kg) reduced the increase of plasma GSSG levels by 54%, totally suppressed the rise of plasma GSH concentrations and increased plasma ALT to 4290 +/- 755 U/l during reperfusion. The data suggest that hepatic glutathione serves to limit ischemia/reperfusion injury as a source of extracellular glutathione, not as a cofactor for the intracellular enzymatic detoxification of reactive oxygen species.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
8755-0199
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12-13 Pt 2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
737-43
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2060845-Animals,
pubmed-meshheading:2060845-Blood Vessels,
pubmed-meshheading:2060845-Carmustine,
pubmed-meshheading:2060845-Extracellular Space,
pubmed-meshheading:2060845-Glutathione,
pubmed-meshheading:2060845-Glutathione Disulfide,
pubmed-meshheading:2060845-Liver,
pubmed-meshheading:2060845-Male,
pubmed-meshheading:2060845-Oxidation-Reduction,
pubmed-meshheading:2060845-Oxygen,
pubmed-meshheading:2060845-Rats,
pubmed-meshheading:2060845-Rats, Inbred F344,
pubmed-meshheading:2060845-Reperfusion Injury
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pubmed:year |
1991
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pubmed:articleTitle |
Vascular oxidant stress and hepatic ischemia/reperfusion injury.
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pubmed:affiliation |
Center for Experimental Therapeutics, Baylor College of Medicine, Houston, Texas 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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