Linked Life Data

20606584

Source:http://linkedlifedata.com/resource/pubmed/id/20606584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-8-2
pubmed:abstractText
The reconstruction of bone defects remains a significant clinical problem. In this study, we constructed cell sheet from bone marrow stromal cells on normal culture plates by a simple method. The cell sheets showed evident mineralized nodules, high alkaline phosphatase activities, indicating their in vitro osteogenic potential. Then its osteogenic capability to heal critical-size rabbit calvarial defect was investigated. Forty adult New Zealand White rabbits were randomly divided into 4 groups of 10 animals each: (1) empty, (2) demineralized bone matrix (DBM) alone, (3) DBM/cell suspension, and (4) DBM/cell sheet. Specimens were harvested 6 and 12 weeks after implantation, respectively. Radiographic, histologic, and histomorphometric analyses were performed to evaluate the new bone formation inside the defect. The results revealed that the defect treated with DBM/cell sheet showed significantly more bone formation than other 3 groups (P < 0.05). Our study indicates that the cell sheet enhances bone regeneration in healing critical-size rabbit calvarial defect, and cell sheet-based engineered bone might be considered as potential substitutes for bone reconstruction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1536-3708
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
259-65
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Reconstruction of rabbit critical-size calvarial defects using autologous bone marrow stromal cell sheets.
pubmed:affiliation
Departments of Oral and Maxillofacial Surgery, Fourth Military Medical University, Shaanxi, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't