Linked Life Data

20604679

Source:http://linkedlifedata.com/resource/pubmed/id/20604679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-5-20
pubmed:abstractText
We studied the effect of permanent unilateral middle cerebral artery occlusion (PMCAO) on the generation of bone marrow (BM)-derived astrocytes in female mice previously transplanted with enhanced green fluorescent protein-expressing BM from male donors. In addition to an untreated PMCAO group, one group of mice also received intracerebral infusion of transforming growth factor-alpha, resulting in a decrease in the size of the infarct. Two months after PMCAO, we found a specific type of astrocyte of BM origin in the side of the injury, near the lesion. These astrocytes did not express glial fibrillary acidic protein (GFAP) by conventional fluorescence immunostaining; however, GFAP was easily detectable by tyramide signal amplification. These cells also expressed S100?, confirming their astrocytic character. Unlike the endogenous reactive astrocytes, these BM-derived astrocytes did not proliferate during the first week of ischemia and did not contribute to the glial scar formation. Transforming growth factor-alpha infusion increased the number of BM-derived astrocytes, without affecting their distribution. Interestingly, exclusively by tyramide signal amplification staining, we found that endogenous astrocytes displaying an identical morphology were also present in control mouse and human brains. Our data demonstrate that a subpopulation of nonreactive astrocytes expressing low levels of GFAP can originate from transplanted BM in the ischemic brain. We believe that these cells represent a subpopulation of astrocytes earlier considered to be GFAP negative. The high number of astrocytes with identical morphology and chemical character in control brains suggest that these type of astrocytes may have important functional role in the central nervous system that calls for further studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1557-8534
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
539-46
pubmed:meshHeading
pubmed-meshheading:20604679-Adult, pubmed-meshheading:20604679-Animals, pubmed-meshheading:20604679-Astrocytes, pubmed-meshheading:20604679-Bone Marrow Cells, pubmed-meshheading:20604679-Bone Marrow Transplantation, pubmed-meshheading:20604679-Brain, pubmed-meshheading:20604679-Brain Ischemia, pubmed-meshheading:20604679-Cell Count, pubmed-meshheading:20604679-Cell Differentiation, pubmed-meshheading:20604679-Disease Models, Animal, pubmed-meshheading:20604679-Female, pubmed-meshheading:20604679-Glial Fibrillary Acidic Protein, pubmed-meshheading:20604679-Humans, pubmed-meshheading:20604679-Infarction, Middle Cerebral Artery, pubmed-meshheading:20604679-Male, pubmed-meshheading:20604679-Mice, pubmed-meshheading:20604679-Nerve Growth Factors, pubmed-meshheading:20604679-Regeneration, pubmed-meshheading:20604679-S100 Proteins, pubmed-meshheading:20604679-Transforming Growth Factor alpha
pubmed:year
2011
pubmed:articleTitle
Bone marrow-derived nonreactive astrocytes in the mouse brain after permanent middle cerebral artery occlusion.
pubmed:affiliation
National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Intramural