Linked Life Data

20602605

Source:http://linkedlifedata.com/resource/pubmed/id/20602605

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-8-2
pubmed:abstractText
Human immunodeficiency virus type 1 (HIV-1) is commonly associated with immune dysfunctions and the suppression of antigen-presenting cells. This results in immune alterations, which could lead to impaired neuronal functions, such as neuroAIDS. The neurotoxic factor kynurenine (KYN), the rate-limiting enzyme indoleamine 2,3-dioxygenase (IDO), serotonin (5-HT), and serotonin transporter (5-HTT) may play a role in tryptophan deficiency and serotogenic dysfunction in neuroAIDS. HIV-1 transactivator regulatory protein (Tat) is known to play a major role in immune dysfunction. Previous studies suggest that HIV-1 B and C clades differentially manifest neuronal dysfunctions in the infected host. In the present study we examine the effect of HIV-1 B and C clade-derived Tat on IDO and 5-HTT gene and protein expressions by dendritic cells as studied by quantitative polymerase chain reaction (qPCR) and Western blot. In addition, the intracellular IDO expression, IDO enzyme activity, and the levels of 5-HT and KYN were also measured. Results indicate that HIV-1 clade B Tat up-regulates IDO and down-regulates 5-HTT gene and protein expressions. Further, HIV-1 clade B Tat caused a reduction of 5-HT with simultaneous increase in KYN levels as compared to HIV-1 clade C Tat. These studies suggest that HIV-1 clade B and C Tat proteins may play a differential role in the neuropathogenesis of HIV-associated dementia (HAD) or HIV-associated neurocognitive disorder (HAND).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1538-2443
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-63
pubmed:dateRevised
2011-3-29
pubmed:meshHeading
pubmed-meshheading:20602605-AIDS Dementia Complex, pubmed-meshheading:20602605-Blotting, Western, pubmed-meshheading:20602605-Cell Separation, pubmed-meshheading:20602605-Dendritic Cells, pubmed-meshheading:20602605-Flow Cytometry, pubmed-meshheading:20602605-Gene Expression, pubmed-meshheading:20602605-Gene Expression Profiling, pubmed-meshheading:20602605-HIV Infections, pubmed-meshheading:20602605-HIV-1, pubmed-meshheading:20602605-Humans, pubmed-meshheading:20602605-Indoleamine-Pyrrole 2,3,-Dioxygenase, pubmed-meshheading:20602605-Kynurenine, pubmed-meshheading:20602605-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20602605-Serotonin, pubmed-meshheading:20602605-Serotonin Plasma Membrane Transport Proteins, pubmed-meshheading:20602605-tat Gene Products, Human Immunodeficiency Virus
pubmed:year
2010
pubmed:articleTitle
Human immunodeficiency virus type 1 clade B and C Tat differentially induce indoleamine 2,3-dioxygenase and serotonin in immature dendritic cells: Implications for neuroAIDS.
pubmed:affiliation
Department of Immunology, Institute of NeuroImmune Pharmacology, College of Medicine, Florida International University, Miami, Florida 33199, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural