Linked Life Data

20602114

Source:http://linkedlifedata.com/resource/pubmed/id/20602114

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-9-8
pubmed:abstractText
Schistosomes are the causative agent of schistosomiasis. The 70-kDa heat-shock proteins (HSP70) are considered the predominant HSP family and play a key regulatory role in parasite development and pathogenesis. Based on the published sequences in Genbank/EMBL, an open-reading frame (ORF) encoding 653 amino acids (XP_002581385.1) and belonging to the Schistosoma HSP70 protein family with a molecular weight of 71.49 kDa was identified by bioinformatic analysis. Since the sequence shared 77% identity with the published full-length Homo sapiens HSP70 protein, it was named Schistosoma mortalin-like protein (MLP/Hsp70). Here, we report the molecular and functional characterization of the Schistosoma japonicum SjMLP/hsp70 as a member of the HSP70 family. The complete SjMLP/hsp70 coding sequence was amplified from a S. japonicum adult worm cDNA library by polymerase chain reaction (PCR) and subcloned into the pET28a expression vector. The purified recombinant protein, rSjMLP/hsp70, was identified as a member of 70-kDa HSP family by mass spectrometry and could be recognized by the S. japonicum-infected mouse serum. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting analysis revealed that SjMLP/hsp70 was widely expressed in the eggs, cercariae, schistosomula, and adult worms of S. japonicum. A thermotolerance assay showed that rSjMLP/hsp70 could protect Escherichia coli cells from heat damage. This chaperone-like activity was demonstrated by full-length SjMLP/hsp70. The detection of specific antibody levels by indirect enzyme-linked immunosorbent assay and IFN-gamma secretion of splenocytes by ELISpot assay suggested that mice immunized with SjMLP/hsp70 were able to elicit Th1-type bias immune response. The challenge-protective experiment showed that DNA vaccine of SjGST combined with SjMLP/hsp70 could induce a 31.31% reduction of worm burden and 58.59% reduction of egg burden in intestinal tissue of immunized mice. Our results imply that SjMLP/hsp70 has a potential adjuvant function and might be a vaccine candidate for schistosomiaisis, which is the first report of the expression and preliminary characterization analysis of this molecule.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1432-1955
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
955-66
pubmed:meshHeading
pubmed-meshheading:20602114-Animals, pubmed-meshheading:20602114-Antibodies, Helminth, pubmed-meshheading:20602114-Blotting, Western, pubmed-meshheading:20602114-Computational Biology, pubmed-meshheading:20602114-DNA, Complementary, pubmed-meshheading:20602114-DNA, Helminth, pubmed-meshheading:20602114-Escherichia coli, pubmed-meshheading:20602114-Female, pubmed-meshheading:20602114-Gene Expression, pubmed-meshheading:20602114-Gene Expression Profiling, pubmed-meshheading:20602114-HSP70 Heat-Shock Proteins, pubmed-meshheading:20602114-HSP72 Heat-Shock Proteins, pubmed-meshheading:20602114-Helminth Proteins, pubmed-meshheading:20602114-Leukocytes, Mononuclear, pubmed-meshheading:20602114-Mass Spectrometry, pubmed-meshheading:20602114-Mice, pubmed-meshheading:20602114-Mice, Inbred BALB C, pubmed-meshheading:20602114-Molecular Chaperones, pubmed-meshheading:20602114-Molecular Weight, pubmed-meshheading:20602114-Rabbits, pubmed-meshheading:20602114-Recombinant Proteins, pubmed-meshheading:20602114-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20602114-Schistosoma japonicum, pubmed-meshheading:20602114-Schistosomiasis japonica, pubmed-meshheading:20602114-Sequence Homology, Amino Acid, pubmed-meshheading:20602114-Vaccination
pubmed:year
2010
pubmed:articleTitle
Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/hsp70) as a member of the HSP70 family.
pubmed:affiliation
Department of Parasitology, Zhongshan School of Medicine, SunYat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't