Source:http://linkedlifedata.com/resource/pubmed/id/20599994
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2011-1-3
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| pubmed:abstractText |
The ribosomal deoxyribonucleic acid (DNA) internal transcribed spacer region (ITS1) of two filarial nematodes, Loa loa and Mansonella perstans, was amplified and further sequenced to develop an species-specific polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol for the differentiation of both species from Wuchereria bancrofti, three filarial nematodes with blood circulating microfilariae. The ITS1-PCR product digested with the restriction endonuclease Ase I generated an specific diagnostic pattern for each of the three species. Moreover, three new specific nested-PCRs, targeting the ITS1 region, for differential detection of L. loa, M. perstans and W. bancrofti were developed and used when the ITS1-PCR products were insufficient for the Ase I enzymatic digestion. These filarial species-specific molecular protocols were evaluated in forty blood samples from African adult immigrants attending in the Hospital Insular of Gran Canaria, Canarias, Spain.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1090-2449
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
127
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
282-6
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| pubmed:meshHeading |
pubmed-meshheading:20599994-Animals,
pubmed-meshheading:20599994-Cloning, Molecular,
pubmed-meshheading:20599994-DNA, Helminth,
pubmed-meshheading:20599994-DNA, Ribosomal,
pubmed-meshheading:20599994-Diagnosis, Differential,
pubmed-meshheading:20599994-Elephantiasis, Filarial,
pubmed-meshheading:20599994-Humans,
pubmed-meshheading:20599994-Loa,
pubmed-meshheading:20599994-Loiasis,
pubmed-meshheading:20599994-Mansonella,
pubmed-meshheading:20599994-Mansonelliasis,
pubmed-meshheading:20599994-Polymerase Chain Reaction,
pubmed-meshheading:20599994-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:20599994-RNA, Helminth,
pubmed-meshheading:20599994-RNA, Ribosomal,
pubmed-meshheading:20599994-Sequence Alignment,
pubmed-meshheading:20599994-Species Specificity,
pubmed-meshheading:20599994-Wuchereria bancrofti
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Detection and discrimination of Loa loa, Mansonella perstans and Wuchereria bancrofti by PCR-RFLP and nested-PCR of ribosomal DNA ITS1 region.
|
| pubmed:affiliation |
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. mjimenez@isciii.es
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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