|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2010-8-3
|
|
pubmed:abstractText |
YB-1 is a member of the cold shock domain family, with complex roles in DNA structure, gene transcription and translation. YB-1 promotes chromosomal instability, and mammary gland transgenic expression induces tumors with 100% penetrance. YB-1 is linked to poor prognosis in breast carcinoma and is a strong predictor of relapse and disease-specific survival. Survival is directly tied to the extent of local invasion and distal metastasis, processes dependent upon the activity of the membrane type I-matrix metalloproteinase, MT1-MMP. Non-invasive MCF-7 breast adenocarcinoma cells were transfected with YB-1/EGFP. YB-1 protein was detected in the invadopodia of cells with a migratory phenotype. There was increased expression of MT1-MMP protein concentrated at the leading edges of motile cells, which were highly invasive in collagen three-dimensional culture. The rates of MT1-MMP protein endocytosis and recycling to the cell surface were elevated in clones expressing higher levels of YB-1 protein. Control MCF-7 cells formed nonfatal, non-invasive, differentiated adenocarcinomas in vivo. MCF-7 cells expressing a twofold increase in YB-1 formed highly anaplastic tumors with local invasion, pulmonary metastases and high lethality. We conclude that YB-1 contributes to the development of an invasive, metastatic breast carcinoma phenotype by enhanced presentation of MT1-MMP at the sites of cellular invasion.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-10504297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-11574481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-11919158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-12604611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-12815724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-12859896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-12915589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-15615704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-15899797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-15985431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-16651443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-17332756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-18793384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-18925950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-19098458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-3174636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-8663054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-9095180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-9278454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20599698-9830047
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1090-2104
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Published by Elsevier Inc.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
30
|
|
pubmed:volume |
398
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
482-8
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:20599698-Adenocarcinoma,
pubmed-meshheading:20599698-Animals,
pubmed-meshheading:20599698-Breast Neoplasms,
pubmed-meshheading:20599698-Cell Line, Tumor,
pubmed-meshheading:20599698-DNA-Binding Proteins,
pubmed-meshheading:20599698-Female,
pubmed-meshheading:20599698-Humans,
pubmed-meshheading:20599698-Matrix Metalloproteinase 14,
pubmed-meshheading:20599698-Mice,
pubmed-meshheading:20599698-Mice, Nude,
pubmed-meshheading:20599698-Neoplasm Invasiveness,
pubmed-meshheading:20599698-Neoplasm Metastasis,
pubmed-meshheading:20599698-Nuclear Proteins,
pubmed-meshheading:20599698-Protein Transport,
pubmed-meshheading:20599698-Y-Box-Binding Protein 1
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
YB-1 alters MT1-MMP trafficking and stimulates MCF-7 breast tumor invasion and metastasis.
|
|
pubmed:affiliation |
The Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California, The Northern California Institute for Research and Education, San Francisco, CA 94121, USA. david.lovett@va.gov
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
