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rdf:type |
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lifeskim:mentions |
umls-concept:C0007137,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0041904,
umls-concept:C0079904,
umls-concept:C0086418,
umls-concept:C0162493,
umls-concept:C0162638,
umls-concept:C0185117,
umls-concept:C0205169,
umls-concept:C1366450,
umls-concept:C1552644,
umls-concept:C1627358,
umls-concept:C1823153,
umls-concept:C1826257,
umls-concept:C2349975,
umls-concept:C2349976,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
2010-7-2
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pubmed:abstractText |
BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-kappaB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-kappaB consensus binding sequence is detected on BAD promoter. Inactivation of NF-kappaB by NF-kappaB inhibitor Bay 11-7082 or NF-kappaB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-kappaB level. Mutation of putative NF-kappaB motif decreased the BAD promoter activity. The binding of NF-kappaB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-kappaB and this may hint of its oncogenic mechanism in cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MLLT11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-Associated Death Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1791-2431
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
547-54
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20596645-Apoptosis,
pubmed-meshheading:20596645-Base Sequence,
pubmed-meshheading:20596645-Blood Proteins,
pubmed-meshheading:20596645-Carcinoma, Squamous Cell,
pubmed-meshheading:20596645-Cell Line, Tumor,
pubmed-meshheading:20596645-Gamma Rays,
pubmed-meshheading:20596645-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:20596645-Humans,
pubmed-meshheading:20596645-Molecular Sequence Data,
pubmed-meshheading:20596645-NF-kappa B,
pubmed-meshheading:20596645-Neoplasm Proteins,
pubmed-meshheading:20596645-Promoter Regions, Genetic,
pubmed-meshheading:20596645-Protein Binding,
pubmed-meshheading:20596645-Proto-Oncogene Proteins,
pubmed-meshheading:20596645-RNA, Small Interfering,
pubmed-meshheading:20596645-Transcription, Genetic,
pubmed-meshheading:20596645-Up-Regulation,
pubmed-meshheading:20596645-bcl-Associated Death Protein
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pubmed:year |
2010
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pubmed:articleTitle |
AF1q enhancement of gamma irradiation-induced apoptosis by up-regulation of BAD expression via NF-kappaB in human squamous carcinoma A431 cells.
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pubmed:affiliation |
Faculty of Medicine, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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