Linked Life Data

20595465

Source:http://linkedlifedata.com/resource/pubmed/id/20595465

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-5-2
pubmed:abstractText
Regions of diminished ventilation are often evident during functional pulmonary imaging studies, including hyperpolarized gas magnetic resonance imaging (MRI), positron emission tomography, and computed tomography (CT). The objective of this study was to characterize the hypointense regions observed via (3)He MRI in a murine model of acute lung injury. LPS at doses ranging from 15-50 ?g was intratracheally administered to C57BL/6 mice under anesthesia. Four hours after exposure to either LPS or saline vehicle, mice were imaged via hyperpolarized (3)He MRI. All images were evaluated to identify regions of hypointense signals. Lungs were then characterized by conventional histology, or used to obtain tissue samples from regions of normal and hypointense (3)He signals and analyzed for cytokine content. The characterization of (3)He MRI images identified three distinct types of hypointense patterns: persistent defects, atelectatic defects, and dorsal lucencies. Persistent defects were associated with the administration of LPS. The number of persistent defects depended on the dose of LPS, with a significant increase in mean number of defects in 30-50-?g LPS-dosed mice versus saline-treated control mice. Atelectatic defects predominated in LPS-dosed mice under conditions of low-volume ventilation, and could be reversed with deep inspiration. Dorsal lucencies were present in nearly all mice studied, regardless of the experimental conditions, including control animals that did not receive LPS. A comparison of (3)He MRI with histopathology did not identify tissue abnormalities in regions of low (3)He signal, with the exception of a single region of atelectasis in one mouse. Furthermore, no statistically significant differences were evident in concentrations of IL-1?, IL-6, macrophage inflammatory protein (MIP)-1?, MIP-2, chemokine (C-X-C motif) ligand 1 (KC), TNF?, and monocyte chemotactic protein (MCP)-1 between hypointense and normally ventilated lung regions in LPS-dosed mice. Thus, this study defines the anatomic, functional, and biochemical characteristics of ventilation defects associated with the administration of LPS in a murine model of acute lung injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1535-4989
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
648-54
pubmed:meshHeading
pubmed-meshheading:20595465-Acute Lung Injury, pubmed-meshheading:20595465-Animals, pubmed-meshheading:20595465-Chemokine CCL2, pubmed-meshheading:20595465-Chemokine CCL3, pubmed-meshheading:20595465-Chemokine CXCL2, pubmed-meshheading:20595465-Escherichia coli, pubmed-meshheading:20595465-Helium, pubmed-meshheading:20595465-Inflammation, pubmed-meshheading:20595465-Interleukin-1beta, pubmed-meshheading:20595465-Interleukin-6, pubmed-meshheading:20595465-Lipopolysaccharides, pubmed-meshheading:20595465-Lung, pubmed-meshheading:20595465-Lung Diseases, pubmed-meshheading:20595465-Magnetic Resonance Imaging, pubmed-meshheading:20595465-Mice, pubmed-meshheading:20595465-Mice, Inbred C57BL, pubmed-meshheading:20595465-Positron-Emission Tomography, pubmed-meshheading:20595465-Tomography, X-Ray Computed, pubmed-meshheading:20595465-Total Lung Capacity, pubmed-meshheading:20595465-Tumor Necrosis Factor-alpha
pubmed:year
2011
pubmed:articleTitle
Ventilation defects observed with hyperpolarized 3He magnetic resonance imaging in a mouse model of acute lung injury.
pubmed:affiliation
National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural