Linked Life Data

2059319

Source:http://linkedlifedata.com/resource/pubmed/id/2059319

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-8-5
pubmed:abstractText
It is reported that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), a physiological factor, has an inductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line (HIMeg) in vitro. At the concentrations ranging from 10(-9) to 10(-6) mol/L, 1,25(OH)2D3 showed inhibition of proliferation on HIMeg cells which was demonstrated by count of survival cells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochemistry (including immunoenzymatic technique) and flow cytometry, we found that HIMeg cells could be further induced into more mature cells in megakaryocytic lineage confirmed by a series of evidence, including the changes of cell morphology/structure and cytochemistry, increased expression of differentiation antigens on the cell surface, and polyploidization. So, it is possible for 1,25(OH)2D3 to promote the differentiation of the cells in megakaryocytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other diseases with malignant megakaryocytosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1001-652X
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
412-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Study on induction of differentiation of a human megakaryoblastic leukemia cell line (HIMeg) by 1,25-dihydroxyvitamin D3.
pubmed:affiliation
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai, PRC.
pubmed:publicationType
Journal Article