Linked Life Data

20586818

Source:http://linkedlifedata.com/resource/pubmed/id/20586818

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-11-16
pubmed:abstractText
Interferon-? (IFN-?) has a direct role in thyroid destruction in autoimmune thyroiditis. Interleukin-18 (IL-18), a pro-inflammatory cytokine with potent IFN-? inducing activities, may play an important role in Th1-mediated autoimmune diseases. The purpose of this study was to characterize the expression and localization of IL-18 in the thyroid tissues of Hashimoto's thyroiditis (HT) and to investigate the effect of IFN-? on IL-18 expression in isolated human thyroid follicular cells (TFCs). Thyroid tissues obtained from six euthyroid patients with HT and six control subjects were used to detect IL-18 expression by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. Human TFCs were isolated and incubated for 48 h with or without IFN-?, tumour necrosis factor-? (TNF-?) or IL-1?. IL-18 expression was analysed by RT-PCR, immunofluorescent double staining and western blot. We found that IL-18 expression was increased in the thyroid tissues of HT compared with control thyroid tissues. TFCs were major cell types expressing IL-18 in the thyroid tissues of HT. IL-18 was constitutively expressed in isolated human TFCs, and the expression was significantly up-regulated by IFN-? rather than TNF-? or IL-1?. Western bolt revealed that a 24-kDa band corresponding to pro-IL-18 was broadened in the lysates of IFN-?-treated TFCs. Our results demonstrated that IL-18 expression is up-regulated in the TFCs of HT patients and in primary human TFCs exposed to IFN-?. Therefore, intrathyroidal interaction between IL-18 and IFN-? may have a role in promoting the local immune response, which contributes to the thyroid destruction seen in HT.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1365-2613
pubmed:author
pubmed:copyrightInfo
© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd.
pubmed:issnType
Electronic
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
420-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Thyrocyte interleukin-18 expression is up-regulated by interferon-? and may contribute to thyroid destruction in Hashimoto's thyroiditis.
pubmed:affiliation
Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't