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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-6-25
pubmed:abstractText
Instruments used for spectral, multispectral, and hyperspectral imaging in the biosciences have evolved significantly over the last 15 years. However, very few are calibrated and have had their performance validated. Now that spectral imaging systems are appearing in clinics and pathology laboratories, there is a growing need for calibration and validation according to universal standards. In addition, some systems produce spectral artifacts that, at the very least, challenge data integrity if left unrecognized. This unit includes a comparison of the band-pass and light-transmission characteristics of electronic tunable filters, interferometers, and wavelength-dispersive spectral imaging instruments, as well as a description of how they work. Methods are described to test wavelength accuracy and perform radiometric calibration. A real-life example of spectral artifacts is dissected in detail in order to show how to detect, diagnose, verify, and work around their presence when they cannot be eliminated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1934-9300
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 12.20
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Approaches to spectral imaging hardware.
pubmed:affiliation
LightForm, Inc., Asheville, North Carolina, USA.
pubmed:publicationType
Journal Article