Linked Life Data

20576621

Source:http://linkedlifedata.com/resource/pubmed/id/20576621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-8-31
pubmed:abstractText
Because of the expansion of the functionalities available for modification of fullerene C60 and its derivatives, their uses are increasing. However, the consequences of fullerene exposure to human health have not been fully studied. In vitro experiments are useful for risk assessment and for understanding potential applications. However, the insolubility of pristine C60 in water makes the in vitro evaluation of cellular responses difficult. To overcome this problem, we prepared a stable and uniform C60-medium dispersion for in vitro examinations. In addition, we examined the effect of the C60-medium dispersion on human keratinocyte HaCaT cells and human lung carcinoma A549 cells to understand the cellular responses induced by exposure to C60. Results indicated that exposure to C60 did not affect cell viability; neither apoptosis nor necrosis were induced, while cell proliferation was inhibited. Furthermore, intracellular oxidative stress was induced by C60 exposure in both HaCaT and A549 cells. Transmission electron microscopy indicated the cellular uptake of C60 aggregates. The results obtained from this study indicate that C60 has oxidative stress induction potential. Further examinations including in vivo studies are necessary for a more accurate evaluation of biological influences by C60.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1756-2651
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-98
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
In vitro evaluation of cellular responses induced by stable fullerene C60 medium dispersion.
pubmed:affiliation
Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. masa-horie@aist.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't