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rdf:type |
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lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0026932,
umls-concept:C0162801,
umls-concept:C0205102,
umls-concept:C0205210,
umls-concept:C0220922,
umls-concept:C0221874,
umls-concept:C0348026,
umls-concept:C0369241,
umls-concept:C0370215,
umls-concept:C0991876,
umls-concept:C1704737,
umls-concept:C2603343
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pubmed:issue |
8
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pubmed:dateCreated |
2010-7-29
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pubmed:abstractText |
The implementation of internal transcribed spacer (ITS) sequencing for routine identification of molds in the diagnostic mycology laboratory was analyzed in a 5-year study. All mold isolates (n = 6,900) recovered in our laboratory from 2005 to 2009 were included in this study. According to a defined work flow, which in addition to troublesome phenotypic identification takes clinical relevance into account, 233 isolates were subjected to ITS sequence analysis. Sequencing resulted in successful identification for 78.6% of the analyzed isolates (57.1% at species level, 21.5% at genus level). In comparison, extended in-depth phenotypic characterization of the isolates subjected to sequencing achieved taxonomic assignment for 47.6% of these, with a mere 13.3% at species level. Optimization of DNA extraction further improved the efficacy of molecular identification. This study is the first of its kind to testify to the systematic implementation of sequence-based identification procedures in the routine workup of mold isolates in the diagnostic mycology laboratory.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-10075499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-11429006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-11462194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-11827268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-12097252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-12230214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-14748802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-15166830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-15872227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-16000456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-16390952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17183689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17191362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17251397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17596364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17634313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17662553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-17710617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-18293184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-18547323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-18642109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-18767206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-18815820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19073865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19112605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19121041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19244469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19375639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19489714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19571014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19930707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-19968730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-2323536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573873-9399543
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1098-660X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2809-13
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pubmed:dateRevised |
2011-7-22
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pubmed:meshHeading |
pubmed-meshheading:20573873-DNA, Fungal,
pubmed-meshheading:20573873-DNA, Ribosomal Spacer,
pubmed-meshheading:20573873-Fungi,
pubmed-meshheading:20573873-Humans,
pubmed-meshheading:20573873-Mycological Typing Techniques,
pubmed-meshheading:20573873-Mycology,
pubmed-meshheading:20573873-Mycoses,
pubmed-meshheading:20573873-Sensitivity and Specificity,
pubmed-meshheading:20573873-Sequence Analysis
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pubmed:year |
2010
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pubmed:articleTitle |
Systematic internal transcribed spacer sequence analysis for identification of clinical mold isolates in diagnostic mycology: a 5-year study.
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pubmed:affiliation |
Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastr. 30/32, 8006 Zürich, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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