Linked Life Data

20573821

Source:http://linkedlifedata.com/resource/pubmed/id/20573821

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2010-8-6
pubmed:abstractText
Following herpes simplex virus type 1 (HSV-1) ocular infection of C57BL/6 mice, activated CD8(+) T cells specific for an immunodominant epitope on HSV-1 glycoprotein B (gB-CD8 cells) establish a stable memory population in HSV-1 latently infected trigeminal ganglia (TG), whereas non-HSV-specific CD8(+) T cells are lost over time. The retention and activation of gB-CD8 cells appear to be influenced by persistent viral antigenic exposure within the latently infected TG. We hypothesized that the low-level expression of gB from its native promoter before viral DNA synthesis is critical for the retention and activation of gB-CD8 cells in the TG during HSV-1 latency and for their ability to block HSV-1 reactivation from latency. To test this, we created a recombinant HSV-1 in which gB is expressed only after viral DNA synthesis from the true late gC promoter (gCp-gB). Despite minor growth differences compared to its rescuant in infected corneas, gCp-gB was significantly growth impaired in the TG and produced a reduced latent genome load. The gCp-gB- and rescuant-infected mice mounted similar gB-CD8 effector responses, but the size and activation phenotypes of the memory gB-CD8 cells were diminished in gCp-gB latently infected TG, suggesting that the stimulation of gB-CD8 cells requires gB expression before viral DNA synthesis. Surprisingly, late gB expression did not compromise the capacity of gB-CD8 cells to inhibit HSV-1 reactivation from latency in ex vivo TG cultures, suggesting that gB-CD8 cells can block HSV-1 reactivation at a very late stage in the viral life cycle. These data have implications for designing better immunogens for vaccines to prevent HSV-1 reactivation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-10438852, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-10790421, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-11602757, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-11773630, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-12551982, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-12753737, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-1312625, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-14633592, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-16051826, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-1654309, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-1683420, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-17229688, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-17360672, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-17522198, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-17579052, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-18005732, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-18619637, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-18845757, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-19073721, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-19158241, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-19188604, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-19349986, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-2839594, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-2846893, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-4357511, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-6283177, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-6321775, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-6480298, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-7853471, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-8207388, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-8670825, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-8673703, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-9188595, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-9420303, http://linkedlifedata.com/resource/pubmed/commentcorrection/20573821-9445016
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8811-20
pubmed:dateRevised
2011-7-25
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Delaying the expression of herpes simplex virus type 1 glycoprotein B (gB) to a true late gene alters neurovirulence and inhibits the gB-CD8+ T-cell response in the trigeminal ganglion.
pubmed:affiliation
Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural