Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-7-31
pubmed:abstractText
The centromere and promoter factor 1 (CPF1) binds specifically in vitro and in vivo to an octanucleotide (RTCACRTG). This sequence is found in the centromere DNA element I (CDEI) of yeast centromeres and upstream from a number of transcription units including MET25, GAL2 and TRP1. Inactivation of the CPF1 gene results in three phenotypes; slow growth, a partial loss of centromere function and methionine auxotrophy. These phenotypes correlate well with the known binding sites for CPF1 and have led to the suggestion that CPF1 functions as a kinetochore protein at centromeres and as a transcriptional activator at promoters such as MET25. By analysing transcription from the MET25, GAL2, and TRP1 genes in cpf1 strains, we demonstrate that CPF1 plays no direct role in their transcriptional regulation. Further evidence in support of this comes from the analysis of point mutations in the basic region of CPF1 that affect DNA binding. A strain expressing a non-DNA bound form of CPF1 is phenotypically Met+, shows normal growth rate but has sub-optimal centromere function. We conclude that a DNA-bound form of CPF1 is required for the kinetochore function but not for maintaining methionine prototrophy.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-1986217, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-1987636, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2115126, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2123466, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2155707, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2185892, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2188087, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2192358, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2249662, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2249772, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2269425, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2338243, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2493990, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2503252, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2543684, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2552290, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2664469, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2668736, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2685569, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2830498, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2835633, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2992949, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2995764, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-2996783, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3011415, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3015730, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3025648, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3027507, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3043181, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3047551, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3082856, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3311877, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3431462, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-347451, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-348684, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3550420, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-3881185, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6159641, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6304646, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6310324, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6329736, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6394957, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-6397474, http://linkedlifedata.com/resource/pubmed/commentcorrection/2057354-7001447
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2961-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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