Source:http://linkedlifedata.com/resource/pubmed/id/20573544
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-9-20
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pubmed:abstractText |
This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (?1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ammonia,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1347-4421
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
110
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
415-8
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pubmed:meshHeading |
pubmed-meshheading:20573544-Ammonia,
pubmed-meshheading:20573544-Base Sequence,
pubmed-meshheading:20573544-Carbon,
pubmed-meshheading:20573544-Culture Media,
pubmed-meshheading:20573544-DNA, Ribosomal,
pubmed-meshheading:20573544-DNA Primers,
pubmed-meshheading:20573544-Glucose,
pubmed-meshheading:20573544-Hydrogen-Ion Concentration,
pubmed-meshheading:20573544-Lysobacter,
pubmed-meshheading:20573544-Nitrogen Fixation,
pubmed-meshheading:20573544-Polymerase Chain Reaction,
pubmed-meshheading:20573544-RNA, Ribosomal, 16S,
pubmed-meshheading:20573544-Temperature
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pubmed:year |
2010
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pubmed:articleTitle |
Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4.
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pubmed:affiliation |
College of Systems Engineering and Science, Department of Bioscience and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama-shi, Saitama 337-8570 Japan.
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pubmed:publicationType |
Journal Article
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