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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-9-2
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pubmed:abstractText |
Inherited skin blistering conditions collectively named epidermolysis bullosa (EB) cause significant morbidity and mortality due to the compromise of the skin's barrier function, the pain of blisters, inflammation, and in some cases scaring and cancer. The simplex form of EB is usually caused by dominantly inherited mutations in KRT5 or KRT14. These mutations result in the production of proteins with dominant-negative activity that disrupt polymerization of intermediate filaments in the basal keratinocyte layer and result in a weak epidermal-dermal junction. The genome of adeno-associated virus (AAV) vectors can recombine with chromosomal sequence so that mutations can be corrected, or production of proteins with dominant-negative activity can be disrupted. We demonstrate a clinically feasible strategy for efficient targeting of the KRT14 gene in normal and EB-affected human keratinocytes. Using a gene-targeting vector with promoter trap design, targeted alteration of one allele of KRT14 occurred in 100% of transduced cells and transduction frequencies ranged from 0.1 to 0.6% of total cells. EBS patient keratinocytes with precise modifications of the mutant allele are preferentially recovered from targeted cell populations. Single epidermal stem cell clones produced histologically normal skin grafts after transplantation to athymic mice and could generate a sufficient number of cells to transplant the entire skin surface of an individual.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-1052771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-11000214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-11152220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-11407989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-11427484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-11996857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-12031128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-12089561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-15208627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-15273747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-15468152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-15776248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-15880606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-16288281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-16824012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-17115047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-1717157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-1720261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-17482546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-17579576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-17712330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-18374450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-18680404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-18931654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-19471246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-2250720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-2497519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-2578890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-2835501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-3041032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-3171211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-6379456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-7537780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-7688405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-8406672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-8500165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-9537413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-9874273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20571545-9882060
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1525-0024
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1624-32
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pubmed:dateRevised |
2011-9-13
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pubmed:meshHeading |
pubmed-meshheading:20571545-Animals,
pubmed-meshheading:20571545-Cells, Cultured,
pubmed-meshheading:20571545-Dependovirus,
pubmed-meshheading:20571545-Epidermolysis Bullosa Simplex,
pubmed-meshheading:20571545-Genetic Vectors,
pubmed-meshheading:20571545-Humans,
pubmed-meshheading:20571545-Immunohistochemistry,
pubmed-meshheading:20571545-Keratin-14,
pubmed-meshheading:20571545-Keratinocytes,
pubmed-meshheading:20571545-Mice,
pubmed-meshheading:20571545-Mice, Nude,
pubmed-meshheading:20571545-NIH 3T3 Cells,
pubmed-meshheading:20571545-Transduction, Genetic
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pubmed:year |
2010
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pubmed:articleTitle |
Efficient KRT14 targeting and functional characterization of transplanted human keratinocytes for the treatment of epidermolysis bullosa simplex.
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pubmed:affiliation |
Department of Pediatrics, Division of Genetic Medicine, University of Washington, Seattle, Washington 98109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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