Source:http://linkedlifedata.com/resource/pubmed/id/20571239
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-6-23
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pubmed:abstractText |
The study was conducted to investigate the effect of Salvianolic acid B (Sal B) on TNF-alpha-stimulated adhesion molecule expression i.e. vascular adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and E-selectin in human aortic endothelial cells (HAECs) under laminar shear stress (LSS) condition. Exposure of HAECs to LSS (12 dynes/cm(2) for 6 h decreased the TNF-alpha-induced protein expression of adhesion molecules i.e. VCAM-1, ICAM-1 and E-selectin. Pre-treatment of HAECs with Sal B (10 microg/ml) then exposed to LSS (12 dynes/cm(2)) for 6 h significantly inhibited VCAM-1, ICAM-1 and E-selectin expression stimulated by TNF-alpha. Moreover, combined Sal B and LSS treatment inhibited the adhesiveness of monocytic U937 cells to TNF-alpha-stimulated HAECs. We further examined the molecular mechanisms and found that the combination of Sal B and LSS treatment dramatically inhibited TNF-alpha-induced NF-kappaB activation evidenced by IkappaBalpha degradation and p65 nuclear translocation in HAECs. This study provides the first biomechanopharmacological evidence that Sal B has a combination effect with LSS to reduce the expression of three adhesion molecules, leading to reduced monocyte adhesion to HAECs, at least in part, by inhibiting the NF-kappaB signaling pathway. Data from this study thus support the potential clinical application of Sal B in vascular inflammatory diseases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/salvianolic acid B
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pubmed:status |
MEDLINE
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pubmed:issn |
1875-8622
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pubmed:author |
pubmed-author:DurairajanSiva Sundara KumarSS,
pubmed-author:HanDongD,
pubmed-author:HuangJian-DongJD,
pubmed-author:KoçLL,
pubmed-author:KooIreneI,
pubmed-author:KumWan FungWF,
pubmed-author:LaoFulongF,
pubmed-author:LiuChen LiCL,
pubmed-author:LuJia HongJH,
pubmed-author:UrenCC,
pubmed-author:WuWei KangWK,
pubmed-author:XieLi XiaLX
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pubmed:issnType |
Electronic
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-58
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pubmed:meshHeading |
pubmed-meshheading:20571239-Anti-Inflammatory Agents,
pubmed-meshheading:20571239-Benzofurans,
pubmed-meshheading:20571239-Cell Adhesion,
pubmed-meshheading:20571239-E-Selectin,
pubmed-meshheading:20571239-Endothelium, Vascular,
pubmed-meshheading:20571239-Humans,
pubmed-meshheading:20571239-Intercellular Adhesion Molecule-1,
pubmed-meshheading:20571239-NF-kappa B,
pubmed-meshheading:20571239-Signal Transduction,
pubmed-meshheading:20571239-Stress, Mechanical,
pubmed-meshheading:20571239-Tumor Necrosis Factor-alpha,
pubmed-meshheading:20571239-U937 Cells,
pubmed-meshheading:20571239-Vascular Cell Adhesion Molecule-1
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pubmed:year |
2010
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pubmed:articleTitle |
The effect of salvianolic acid B combined with laminar shear stress on TNF-alpha-stimulated adhesion molecule expression in human aortic endothelial cells.
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pubmed:affiliation |
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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