Linked Life Data

20564491

Source:http://linkedlifedata.com/resource/pubmed/id/20564491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-11-24
pubmed:abstractText
The thorns of Gleditsia sinensis are used as a medicinal herb in China and Korea. However, the mechanisms responsible for the antitumor effects of the water extract of Gleditsia sinensis thorns (WEGS) remain unknown. HCT116 cells treated with the WEGS at a dose of 800??g/mL (IC??) showed a significant decrease in cell growth and an increase in cell cycle arrest during the G2/M-phase. G2/M-phase arrest was correlated with increased p53 levels and down-regulation of the check-point proteins, cyclinB1, Cdc2 and Cdc25c. In addition, treatment with WEGS induced phosphorylation of extracellular signal-regulated kinase (ERK), p38 MAP kinase and JNK (c-Jun N-terminal kinases). Moreover, inhibition of ERK by treatment of cells with the ERK-specific inhibitor PD98059 blocked WEGS-mediated p53 expression. Similarly, blockage of ERK function in the WEGS-treated cells reversed cell-growth inhibition and decreased cell cycle proteins. Finally, in vivo WEGS treatment significantly inhibited the growth of HCT116 tumor cell xenografts in nude mice with no negative side effects, including loss of body weight. These results describe the molecular mechanisms whereby the WEGS might inhibit proliferation of colon cancer both in vitro and in vivo, suggesting that WEGS has potential as an anticancer agent for the treatment of malignancies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1099-1573
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1870-6
pubmed:meshHeading
pubmed-meshheading:20564491-Animals, pubmed-meshheading:20564491-Cell Cycle, pubmed-meshheading:20564491-Cell Cycle Proteins, pubmed-meshheading:20564491-Cell Line, Tumor, pubmed-meshheading:20564491-Cell Proliferation, pubmed-meshheading:20564491-Colonic Neoplasms, pubmed-meshheading:20564491-Down-Regulation, pubmed-meshheading:20564491-Drugs, Chinese Herbal, pubmed-meshheading:20564491-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:20564491-Flavonoids, pubmed-meshheading:20564491-Gene Expression Regulation, Neoplastic, pubmed-meshheading:20564491-Gleditsia, pubmed-meshheading:20564491-Humans, pubmed-meshheading:20564491-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:20564491-Mice, pubmed-meshheading:20564491-Mice, Nude, pubmed-meshheading:20564491-Plant Extracts, pubmed-meshheading:20564491-Tumor Suppressor Protein p53, pubmed-meshheading:20564491-Xenograft Model Antitumor Assays, pubmed-meshheading:20564491-p38 Mitogen-Activated Protein Kinases
pubmed:year
2010
pubmed:articleTitle
Gleditsia sinensis thorn extract inhibits human colon cancer cells: the role of ERK1/2, G2/M-phase cell cycle arrest and p53 expression.
pubmed:affiliation
Department of Biotechnology, Chungju National University, Chungju, Chungbuk 380-702, South Korea; Department of Food Science and Technology, Chung-Ang University, Ansung 72-1, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't