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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-8-1
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pubmed:abstractText |
We have used immunocytochemistry to identify the major primary adhesion molecule of the cadherin class in human kidney. In frozen sections of kidney, A-CAM was detected using the monoclonal antibody GC4 on the surface of renal tubular epithelial cells. Renal tubular epithelium did not express L-CAM. No cadherin reactivity was found on the glomerular epithelial cells. Cultured renal tubular epithelium was studied by immunofluorescence and immunogold methods. A-CAM was found at the contact points of adjacent epithelial cells, the phenotype of which was confirmed by the demonstration of cytokeratins using the antibody CAM 5.2. The A-CAM molecule in human kidney had an Mr of 130 kD in Western blotting experiments. These results lead us to conclude that A-CAM is the major cadherin of adult human renal epithelium.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/CDH2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-3417
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-15
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2056393-Antibodies, Monoclonal,
pubmed-meshheading:2056393-Antigens, CD,
pubmed-meshheading:2056393-Blotting, Western,
pubmed-meshheading:2056393-Cadherins,
pubmed-meshheading:2056393-Cell Adhesion,
pubmed-meshheading:2056393-Cell Adhesion Molecules,
pubmed-meshheading:2056393-Cells, Cultured,
pubmed-meshheading:2056393-Epithelial Cells,
pubmed-meshheading:2056393-Epithelium,
pubmed-meshheading:2056393-Fluorescent Antibody Technique,
pubmed-meshheading:2056393-Humans,
pubmed-meshheading:2056393-Kidney
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pubmed:year |
1991
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pubmed:articleTitle |
Morphological evidence that A-CAM is a major intercellular adhesion molecule in human kidney.
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pubmed:affiliation |
Department of Pathology, University of Edinburgh, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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