Source:http://linkedlifedata.com/resource/pubmed/id/20561442
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2010-6-21
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pubmed:abstractText |
This study was aimed to investigate the effects of bortezomib on VEGF gene expression of endothelial cell line HMEC-1, and to determine the changes of the transcriptional regulation activity of hypoxia-inducible factor 1 (HIF-1alpha) and expression intensity of Annexin A2, so as to analyze the possible mechanisms of the above expression of VEGF gene. Expression intensity of VEGF gene was determined by real-time quantitative PCR; the relative proliferation activity of cells was assayed by cell count kit CCK-8; the expression intensity of carbonic anhydrase IX (CA IX) gene was detected by RT-PCR; expression of Annexin A2 at gene and protein levels were determined by real-time quantitative PCR and Western blot respectively. The results showed that after being treated by bortezomib with 2.5, 5.0, 10 nmol/L for 12 hours, the expression intensity of VEGF gene of endothelial cell line HMEC-1 was as follows: 0.730 +/- 0.106, 0.673 +/- 0.153, 0.767 +/- 0.090 (as 1.0 was made in 0 nmol/L) (p < 0.05); the proliferation activity of cells was not significantly suppressed by bortezomib in 2.5, 5.0 nmol/L (p > 0.05), while that was significantly suppressed by bortezomib of 10 nmol/L (p = 0.024), The results from RT-PCR showed that expression intensity of CA IX gene was conspicuously down-regulated by bortezomib in different concentrations, which suggested that the transcriptional regulation activity of HIF-1alpha was inhibited by bortezomib. And down-regulated expression of Annexin A2 protein by bortezomib in different concentrations was confirmed by real-time quantitative PCR and Western blot. It is concluded that low doses of bortezomib has no significant inhibition effect on the activity of proteasome. Bortezomib may down-regulate the expression of VEGF gene of endothelial cell through regulating the activity of HIF-1alpha and the expression of Annexin A2.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Boronic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/CA9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonic Anhydrases,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazines,
http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/bortezomib
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1009-2137
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
744-8
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pubmed:meshHeading |
pubmed-meshheading:20561442-Annexin A2,
pubmed-meshheading:20561442-Antigens, Neoplasm,
pubmed-meshheading:20561442-Boronic Acids,
pubmed-meshheading:20561442-Carbonic Anhydrases,
pubmed-meshheading:20561442-Cell Line,
pubmed-meshheading:20561442-Down-Regulation,
pubmed-meshheading:20561442-Endothelial Cells,
pubmed-meshheading:20561442-Gene Expression,
pubmed-meshheading:20561442-Humans,
pubmed-meshheading:20561442-Hypoxia-Inducible Factor 1, alpha Subunit,
pubmed-meshheading:20561442-Pyrazines,
pubmed-meshheading:20561442-Vascular Endothelial Growth Factor A
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pubmed:year |
2010
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pubmed:articleTitle |
[Effect of bortezomib on VEGF gene expression of endothelial cell line HMEC-1 and its possible mechanisms].
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pubmed:affiliation |
Jiangsu Institute of Hematology, Suzhou University First Affiliated Hospital, Key Laboratory of Thrombosis and Hemostasis Subordinate to Ministry of Health, Suzhou 215006, Jiangsu Province, China.
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pubmed:publicationType |
Journal Article,
English Abstract
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