Linked Life Data

20560532

Source:http://linkedlifedata.com/resource/pubmed/id/20560532

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2010-7-29
pubmed:abstractText
A novel cyanide analyzer based on sensitive cobinamide chemistry relies on simultaneous reagent and sample injection and detection in a 50 cm liquid core waveguide (LCW) flow cell illuminated by a white light emitting diode. The transmitted light is read by a fiber-optic charge coupled device (CCD) spectrometer. Alkaline cobinamide (orange, lambda(max) = 510 nm) changes to violet (lambda(max) = 583 nm) upon reaction with cyanide. Multiwavelength detection permits built-in correction for artifact responses intrinsic to a single-line flow injection system and corrects for drift. With optimum choice of the reaction medium, flow rate, and mixing coil length, the limit of detection (LOD, S/N = 3) is 30 nM and the linear dynamic range extends to 10 microM. The response base width for 1% carryover is <95 s, permitting a throughput of 38 samples/h. The relative standard deviations (rsd) for repetitive determinations at 0.15, 0.5, and 1 microM were 7.6% (n = 5), 3.2% (n = 7), and 1.7% (n = 6), respectively. Common ions at 250-80,000x concentrations do not interfere except for sulfide. For the determination of 2 microM CN(-), the presence of 2, 5, 10, 20, 100, and 1000 microM HS(-) results in 22, 27, 48, 58, 88, and 154% overestimation of cyanide. The sulfide product actually has a different characteristic absorption, and in those samples where significant presence is likely, this can be corrected for. We demonstrate applicability by analyzing the hydrolytic cyanide extract of apple and pear seeds with orange seeds as control and also measure HCN in breath air samples. Spike recoveries in these sample extracts ranged from 91 to 108%.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-12935566, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-14632117, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-15632180, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-17037934, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-17716310, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-18965596, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-19480566, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-19842647, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-20023843, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-20302333, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-20420400, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-2837167, http://linkedlifedata.com/resource/pubmed/commentcorrection/20560532-3233193
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1520-6882
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6244-50
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Cobinamide-based cyanide analysis by multiwavelength spectrometry in a liquid core waveguide.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of Texas, 700 Planetarium Place, Arlington, Texas 76019-0065, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural